Technology and Development Team for Mammalian Cellular Dynamics

Team Leader
Kuniya ABE (Ph.D.)
We develop technologies to monitor and to analyze dynamic nature of mammalian cells, which will be useful tools for phenotyping biological resources collected at the BioResource Center. In order to achieve this goal, our team uses genetic, genomic and transgenic/gene targeting approaches. Our focus is in the following areas. 1. Visualization and purification of pluripotent embryonic cells and germ cells, and genome wide analysis of gene expression to understand genomic reprogramming processes. 2. Identification and functional characterizations of genes required for mammalian early development through the analysis of t-complex mutant mice. 3. Novel research technique to analyze expression of non-coding RNAs during development. 4. Functional genomic analysis using BAC transgenesis technology 5. Evaluations of functional processes in live animals by in vivo imaging
- Visualization and purification of pluripotent embryonic cells and germ cells, and genome wide analysis of gene expression to understand genomic reprogramming processes.
- Identification and functional characterizations of genes required for mammalian early development through the analysis of t-complex mutant mice.
- Novel research technique to analyze expression of non-coding RNAs during development.
- Functional genomic analysis using BAC transgenesis technology
- Evaluations of functional processes in live animals by in vivo imaging
- September ,2010
- Near tenfold improvement achieved in mouse somatic cell clone birthrate
- March 23,2010
- Experimental mouse evolves in its feeding place and reaches maturity early without secreting melatonin
- Inoue, K., Kohda, T., Sugimoto, M.et al.:
"Impeding Xist expression from the active X chromosome improves mouse somatic cell cloning."
Science 330, 496-499(2010). - Honda, A., Hirose, M., Inoue, K., Hiura, H., Miki, H., Ogonuki, N., Sugimoto, M.Abe, K., Kanatsu-Shinohara, M., Kono, T., Shinohara, T. and Ogura, A.,:
"Genetic variation of melatonin productivity in laboratory mice under domestication."
Proc. Natl. Acad. Sci, USA 107, 6412-6417 (2010). - Kasahara, T., Abe, K., Mekada, K., Yoshiki, A., Kato, T.:
"The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos."
Nucleic Acids Res. 2010 Feb.25. [Epub ahead of print] - Cao L, Shitara H, Sugimoto M, Hayashi J, Abe K, Yonekawa H.:
"New evidence confirms that the mitochondrial bottleneck is generated without reduction of mitochondrial DNA content in early primordial germ cells of mice."
PLoS Genetics 5, e1000756. (2009). - Tomida S, Mamiya T, Sakamaki H, Miura M, Aosaki T,Masuda M, Niwa M, Kameyama T, Kobayashi J, Iwaki Y, Imai S, Ishikawa A, Abe K, Yoshimura T, Nabeshima T and Ebihara S.:
"Usp46 is a quantitative trait gene regulating mouse immobile behavior in the tail suspension and forced swimming tests"
Nature Genetics 41, 688-695 (2009) - Mise, N., Fuchikami, T., Sugimoto, M., Kobayakawa, S., Ike, F., Ogawa, T., Tada, T., Kanaya, S., Noce, T.Abe, K.:
"Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling."
Genes to Cells 13, 863-877 (2008). - Sugimoto, M. and Abe, K.:
"X chromosome reactivation initiates in nascent primordial germ cells in mice."
PLoS Genetics 3, e116 (2007). - Kobayakawa, S., Miike, K., Nakao, M. and Abe, K.:
"Dynamic Changes in the Epigenomic State and Nuclear Organization of Differentiating Mouse Embryonic Stem Cells."
Genes to Cells 12, 447-460 (2007). - Cao, L., Shitara, H., Horii, T., Nagao, Y., Imai, H., Abe, K., Hara, T., Hayashi, J.-I., Yonekawa, H.:
"Generation of the mitochondrial bottleneck without the reduction of mtDNA content in mouse female germ cells"
Nature Genetics 39, 386-390 (2007). - Kobayashi, S., Isotani, A., Mise, N., Yamamoto, M., Fujihara, Y., Kaseda, K., Nakanishi, T., Ikawa, M., Hamada, H., Abe, K. and Okabe, M.:
"Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the X-linked gene, Rhox5/Pem, at preimplantation stages."
Current Biology 16, 166-172 (2006).
Principal Investigator
- Kuniya ABE
- Team Leader
Staff Scientist
- Michihiko SUGIMOTO
- Research and Development Scientist
- Koji NUMATA
- Research and Development Scientist
Postdoctoral Fellow
- Liqin CAO
- Foreign Postdoctoral Researcher
- Hirosuke SHIURA
- Postdoctoral Researcher
Technical Assistant
- Masayo KONDO
- Technical Staff 2
- Rieko IKEDA
- Technical Staff 2
- Yumiko KOGA
- Technical Staff 2