Technology and Development Team for Mouse Phenotype Analysis

Team Leader
Shigeharu WAKANA (Ph.D.)
The Technology and Development Team for Mouse Phenotype Analysis develop a systematic and comprehensive phenotyping platform consisting of more than 400 test items, based on the knowledge of pathogenesis of human diseases. Using this system, we perform more comprehensive and profound phenotyping of mouse mutants, which are and will be deposited to RIKEN BRC. Judging from the results of preceding foreign phenotyping projects, we expect that existing mouse mutants be revalued as new disease models by identified novel phenotypes. We also develop a standard operating procedures (SOP) of mouse phenotyping and exchange this information with other large-scale related projects, such as EUMODIC (European Mouse Disease Clinic) and MPP (Mouse Phenome Project), and thereby we will contribute to international efforts for standardization of mouse phenotype data. Finally, through collaborations with and our team will integrate information of mouse phenotypes and clinical features of human diseases. It will enable more objective characterization of mouse mutants, and lead to the development of high value-added mouse resources.
- Development of a systematic and comprehensive mouse phenotyping platform.
- Development of standard operating procedures (SOP) of mouse phenotyping
- Collaboration with the large-scale mouse phenotyping projects, such as EUMODIC (European Mouse Disease Clinic) and MPP (Mouse Phenome Project).
- The development of high value-added mouse resources corresponding with human disease information
- Wakana, S., Suzuki, T., Furuse, T., Kobayashi, K., Miura, I., Kaneda, H., Yamada, I., Motegi, H., Toki, H., Inoue, M., Minowa, O., Noda, T., Waki, K., Tanaka, N., Masuya, H.,and Obata, Y.:
"Introduction to the Japan Mouse Clinic at the RIKEN BioResource Center."
Exp Anim. 58(5):443-450 (2009) - Taguma, K., Nakamura, C., Ozaki, A., Suzuki, C., Hachisu, A., Kobayashi, K., Mochida, K., Ogura, A., Kaneda, H.,and Wakana.:
"A practical novel method for ensuring stable capacitation of spermatozoa from cryopreserved C57BL/6J sperm suspension."
Exp Anim. 58(4):395-401 (2009) - Ogonuki, N., Inoue, K., Hirose, M., Miura, I., Mochida, K., Sato, T., Mise, N., Mekada, K., Yoshiki, A., Abe, K., Kurihara, H., Wakana, S.,and Ogura, A.:
"A high-speed congenic strategy using first-wave male germ cells."
PLoS One4(3):e4941 (2009) - Masuya, H., Yoshikawa, S., Heida, N., Toyoda, T., Wakana, S.,and Shiroishi, T.:
"PHENOSITE: A web database integrating the mouse phenotyping platform and the experimental procedures in mice."
Journal of Bioinformatics and Computational Biology, 5(6): 1173-1191 (2007) - Kaneda, H., Taguma, K., Suzuki, C., Ozaki, A., Nakamura, C., Hachisu, A., Kobayashi, K., Wakana, S.,and Shiroishi, T.:
"An Optimal Embryo Transfer Condition for the Effective Production of DBA/2J Mice."
Experimental Animals, 56(5): 385-388 (2007) - Masuya, H., NishidaK., Furuichi, T., Toki, H., Nishimura, G., Kawabata, H., Yokoyama, H., Yoshida, A., Tominaga, S., Nagano, J., Shimizu, A., Wakana, S., Gondo, Y., Noda, T., Shiroishi, T.,and Ikegawa, S.:
"A novel dominant-negative mutation in Gdf5 generated by ENU mutagenesis impairs joint formation and causes osteoarthritis in mice."
Hum Mol Genet., 16: 2366-2375 (2007) - Masuya, H., Inoue, M., Wada, Y., Shimizu, A., Nagano, J., Kawai, A., Inoue, A., Kagami, T., Hirayama, T., Yamaga, A., Kaneda, H., Kobayashi, K., Minowa, O., Miura, I., Gondo, Y., Noda, T., Wakana, S.,and Shiroishi, T.:
"Implementation of Modified-SHIRPA protocol for screening of dominant phenotypes in a large-scale ENU mutagenesis program."
Mammalian Genome, 16(11): 829-837 (2005) - Masuya, H., Shimizu, K., Sezutsu, H., Sakuraba, Y., Nagano, J., Shimizu, A., Fujimoto, N., Kawai, A., Miura, I., Kaneda, H., Kobayashi, K., Ishijima, J., Maeda, T., Gondo, Y., Noda, T., Wakana, S.,and Shiroishi, T.:
"Enamelin (Enam) is essential for amelogenesis ENU-induced mouse mutants as models for different clinical subtypes of human amelogenesis imperfecta (AI)."
Hum Mol Genet., 14: 575-583 (2005) - Inoue, M., Sakuraba, Y., Motegi, H., Kubota, N., Toki, H., Matsui, J., Toyoda, Y., Miwa, I., Terauchi, Y., Kadowaki. T., Shigeyama, Y., Kasuga, M., Adachi, T., Fujimoto, N., Matsumoto, R., Tsuchihashi, K., Kagami, T., Inoue, A., Kaneda, H., Ishijima, J., Masuya, H., Suzuki, T., Wakana, S., Gondo, Y., Minowa, O., Shiroishi, T.,and Noda, T.:
"A series of maturity onset diabetes of the young, type 2 (MODY2) mouse models generated by a large-scale ENU mutagenesis program."
Hum Mol Genet., 13(11): 1147-1157 (2004) - Masuya, H., Nakai, Y., Motegi, H., Niinaya, N., Kida, Y., Kaneko, Y., Aritake, H., Suzuki, N., Ishii, J., Koorikawa, K., Suzuki, T., Inoue, M., Kobayashi, K., Toki, H., Wada, Y., Kaneda, H., Ishijima, J., Takahashi, KR., Minowa, O., Noda, T., Wakana, S., Gondo, Y.,and Shiroishi, T.:
"Development and implementation of a database system to manage a large-scale mouse ENU-mutagenesis program."
Mammalian Genome, 15: 404-411 (2004)
Principal Investigator
- Shigeharu WAKANA
- Team Leader
Staff Scientist
- Hideki KANEDA
- Research and Development Scientist
- Tamio FURUSE
- Research and Development Scientist
- Tomohiro SUZUKI
- Research and Development Scientist
- Kimio KOBAYASHI
- Technical Scientist
- Ikuo MIURA
- Technical Scientist
- Ikuko YAMADA
- Technical Scientist
Technical Assistant
- Tomoko KUSHIDA
- Technical Staff 2
- Kyoko IKEDA
- Technical Staff 2
- Ai OZAKI
- Technical Staff 2
- Chigusa SUZUKI
- Technical Staff 2
- Chika NAKAMURA
- Technical Staff 2
- Akiko SHINOGI
- Technical Staff 2
- Yasuyo KOZAWA
- Technical Staff 2