Genetic Engineering Unit
Unit Leader
Shinichi AIZAWA (Ph.D.)
Quality and efficacy of the research are greatly dependent on how easily mutant mice are handled. The major aim of this laboratory is to develop mutant mice useful for researches in Developmental Biology and Regenerative Medicinebyin collaboration with researchers not only in CDB but also in Japan and Asian. With the sequence information on genes from researchers, the construction of vectors, ES culture, isolation of homologous recombinants, production of chimera as well as generation of transgenic miceare all being done speedily by this laboratory. Special focus is in development of mutant mice, embry oculture and microscopic techniques for bioimaging in mouse embryos.
- Generation of mouse mutants useful for developmental biology and regenerative medicine
- Development of mutant mice and techniques for bioimaging in mouse embryos
- Development of technology to manipulate mouse embryos genetically and embryologically
- Shahidul M.Islam, Yohei Shinmyo, Tatsuya Okafuji, Yuhong Su, Iftekhar Bin Nase, Giasuddin Ahmed, Sanbing Zhang, Sandy Chen, Kunimasa Ohta, Hiroshi Kiyonari, Takaya Abe, Satomi Tanaka, Ryuichi Nishinakamura, Toshio Terashima, Toshio Kitamura, Hideaki Tanaka.:
"Draxin, a Repulsive Guidanc Protein for Spinal Cord and Forebrain Commissures."
Science 323, 388-393(2009). - Imai, T., Yamazaki, T., Kobayakawa, R., Abe, T., Suzuki, M., and Sakano, H.:
"Pre-target axon sorting establishes the neural map topography."
Science 325, 585-590(2009). - Ohno, M., Hiraoka, Y., Matsuoka, T., Tomimoto, H., Takao, K., Miyakawa, T., Oshima, N., Kiyonari, H., Kimura, T., Kita, T. and Nishi, E.:
"Nardilysin regulates axonal maturation and myelination in the central and peripheral nervous system."
Nature Neuroscience 12, 1506-1513(2009). - Uchiyama, Y., Skaguchi, M., Terabayashi, T., Inenaga, T., Inoue, S., Kobayashi, C., Oshima, N., Kiyonari, H., Nakagata, N., Sato, Y., Sekiguchi, K., Miki, H., Araki, E., Fujimura, S., Tanaka, S, S., Nishinakamura, R.:
"Kif26b, a kinesin family gene, regulates adhesion of the embryonic kindney mesenchyme."
PNAS. 107, 9240-9245(2010). - Kiyonari, H., Kaneko, M., Abe, S. and Aizawa, S.:
"Three Inhibitors of FGF Receptor, ERK, and GSK3 Establishies Germline-Competent Embryonic Stem Cells of C57BL/6N Mouse Strain With High Efficiency and Stability."
Genesis. 48, 317-327(2010). - Yamanishi Y, Kitaura J, Izaawa K, Kitani A, Komeno Y, Nakamra M, Yamazaki S, Enomoto Y, Oki T, Akiba H, Abe T, Komori T, Morikawa Y, Kiyonari H, Takai T, Okumura K, Kitamura T.
"TIM1 is an endogenous ligand for LMIR5/CD300b:LMIR5 deficiency ameliorates mouse kidney ischemia/reperfusion injury."
JEM 207, 1501-1511(2010). - Sato, S., Ikeda, K., Shioi, G., Ochi, H., Ogino, H., Yajima, H., Kawakami, K.:
"Conserved expression of mouse Six1 in the pre-placodal region(PPR) and identification of an enhancer for the rostral PPR."
Developmental Biology 344, 158-171(2010). - Kumada T, Yamanaka Y, Kitano A, Shibata M, Awaya T, Kato T, Okawa K, Abe T, Oshima N, Nakahata T, Heike T.:
"Ttyh1, a Ca2+-Binding Protein Localized to the Endoplasmic Reticulum, Is Required for Early Embryonic Development."
DEVELOPMENTAL DYNAMICS 239, 2233-2245(2010). - Oboki K, Ohno T, Kajiwara N, Arae K, Morita H, Ishii A, Nambu A, Abe T, Kiyonari H, Matsumoto K, Sudo K, Okumura K, Saito H, Nakae S.:
"IL-33 is a crucial amplifier of innate rather than acquired immunity."
Proc Natl Acad Sci U S A. 107(43), 18581-18586(2010). - Tao H, Manak R, Sowers L, Mei X, Kiyonari H, Abe T, Dahdaleh NS, Yang T, Wu S, Chen S. Fox MH, Gurnett C. Montine T, Bird T, Shaffer LG, Rosenfeld JA, McConnell J, Madan-Khetarpal S, Berry-Kravis E, Griesbach H, Saneto R, Scott MP, Antic D, Reed J, Boland R, Ehaideb SN, El-Shanti H, Mahajan VB, Ferguson PJ, Axelrod JD, Lehesjoki AE, Fritzsch B, Slusarski DC, Wemmie J, Ueno N. Bassuk AG.:
"Mutations in Prickle Orthologs Cause Seizures in Flies, Mice, and Humans."
The American Journal of Human Genetics 88, 1-12(2011).