Plant Immunity Research Team
Research Areas
| Our ultimate goal is to fully describe functions of genes, proteins and small molecular compounds that are essential for immunity in plants. As the first step, we focus on regulatory mechanism of immunity by studying dynamics of resistance signaling complexes and protein modifications that control defense responses. In addition, we plan to identify novel genes involved in plant immunity by isolating defense mutants in model plants. We also collaborate with the Metabolomics Research Group to isolate small molecule compounds involved in disease resistance. |  Team Leader Ken SHIRASU (Ph.D.)  |
Research Subjects
| (1) | Characterization of plant immunity signaling complexes |
| (2) | Characterization of ubiquitin ligases involved in plant immunity |
| (3) | Isolation and characterization of novel genes in plant immunity by chemical genetics |
| (4) | Plant immunity studies by proteomics |
| (5) | Molecular analysis of parasitic plant Striga |
List of Selected Publications
| (1) | Shirasu, K.: "The HSP90-SGT1 Chaperone Complex for NLR Immune Sensors." Annual Review of Plant Biology 60 (2009) |
| (2) | Kadota, Y., Amigues, B., Ducassou, L., Madaoui, H., Ochsenbein, F., Guerois, R. and Shirasu, K.: "Structural and functional analysis of SGT1-HSP90 core complex required for innate immunity in plants." EMBO reports 9:1209-1215 (2008) |
| (3) | Trujillo, M., Ichimura, K., Casais, C., and Shirasu, K.: "Negative regulation of PAMP-triggered immunity by E3 ubiquitin ligase triplet in Arabidopsis." Current Biology 18:1396-1401. (2008) |
| (4) | Umehara, M., Hanada, A., Yoshida, S., Akiyama, K., Arite, T., Takeda-Kamiya, N., Magome, H., Kamiya, Y., Shirasu, K., Yoneyama, K., Kyozuka, J., and Yamaguchi, S.: "Inhibition of shoot branching by new terpenoid plant hormones." Nature 455: 195-200. (2008) |
| (5) | Sugiyama, N., Nakagami, H., Mochida, K., Daudi, A., Tomita, M., Shirasu, K., and Ishihama, Y.: "Large-scale phosphorylation mapping reveales the extent of tyrosine phosphorylation in Arabidopsis." Molecular Systems Biology 4:193 (2008) |
| (6) | Boter, M., Amigues, B., Peart, J., Breuer, C., Kadota, Y., Casais, C., Moore, G., Kleanthous, C., Ochsenbein, F., Shirasu, K., and Guerois, R.: "Structural and functional analysis of SGT1 reveals that its interaction with HSP90 is required for the accumulation of Rx, an R protein involved in plant immunity." Plant Cell 19: 3791-3804. (2007) |
| (7) | Maor, R., Jones, A., Nuhse, T.S., Studholme, D.J., Peck, S.C., and Shirasu, K.: "MudPIT analysis of ubiquitinated proteins in plants." Molecular & Cellular Proteomics 6: 601-610. (2007) |
| (8) | Azevedo, C., Betsuyaku, S., Peart, J., Takahashi, A., Noel, L., Sadanandom, A., Casais, C., Parker, J., and Shirasu, K.: "Role of SGT1 in resistance protein accumulation in plant immunity." EMBO J. 25: 2007-2016. (2006) |
| (9) | Takahashi, A., Casais, C., Ichimura, K., and Shirasu, K.: "HSP90 interacts with RAR1 and SGT1, and is essential for RPS2-mediated disease resistance in Arabidopsis." Proc. Acad. Natl. Sci. U.S.A.100: 11777-11782. (2003) |
| (10) | Azevedo, C., Sadanandom, A., Kitagawa, K., Freialdenhoven, A., Shirasu, K., and Schulze-Lefert, P.: "The RAR1 interactor SGT1, an essential component of R-gene triggered disease resistance." Science 295: 2073-2076.(2002) |