Laboratory for Lymphocyte Development
Team Leader
Hiroshi KAWAMOTO
(M.D., Ph.D.)
The goal of our project is to clarify the process of lineage restriction from multipotent hematopoietic stem cells to unipotent progenitors, and to understand the molecular mechanisms that regulate the cell fate decisions. We have previously established a clonally assay system which can examine the developmental potential of individual progenitors toward T, B and myeloid cell lineages (multilineage progenitor assay; MLP asay). Based on the findings obtained by this method and by some modified methods, we have proposed a novel model of lineage restriction pathway in hamatopoiesis. We are now trying to develop a new real-time monitoring system for the lineage restriction process, which will make it possible to study the mechanism that drives differentiation program in hamatopoiesis. The information and technology which will be acquired through this project can directly be applied to the regeneration therapy and gene therapy utilizing hematopoitic stem/progenitor cells, and will also contribute to the elucidation of mechanisms in neoplastic development of blood cells.
- Prethymic pathway of T cell development
- Early stages of T cell development in the thymus
- Process of lineage restriction of the hematopoietic stem cell
- July 2, 2010
- The last checkpoint to T cell fate
- April 10, 2008
- A close relationship between T cells and phagocytes
- September 03, 2010
- Crossing the line
Understanding of blood cell lineages advances with the discovery of a transcription factor crucial to T cell differentiation
- Ikawa T, Hirose S, Masuda K, Kakugawa K, Satoh R, Shibano-Satoh A, Kominami R, Katsura Y, Kawamoto H.
"An essential developmental checkpoint for production of the T cell lineage."
Science. 329: 93-96 (2010) - Moro K., T. Yamada, M. Tanabe, T. Takeuchi, T. Ikawa, H. Kawamoto, J-I. Furusawa, M. Ohtani, H. Fujii, and S. Koyasu:
"Innate production of Th2 cytokines by adipose tissue-associated c-Kit+Sca-1+ lymphocytesimmunoglobulin-like receptors"
Nature 463: 540-544 (2010) - Kakugawa K., T. Yasuda, I. Miura, A. Kobayashi, H. Fukiage, R. Satoh, M. Matsuda, H. Koseki, S. Wakana, H. Kawamoto, H. Yoshida
"A novel gene essential for the development of single positive thymocytes"
Mol. Cell. Biol. 29:5128-5135 (2009) - Kawamoto, H. and Katsura, Y.
"A new paragigm for hematopoietic cell lineages: revision of the classical concept of the myeloid-lymphoid dichomoty"
Trends in Immunology. 30: 193-200 (2009) - Muroi, S., Y Naoe, C Miyamoto, K Akiyama, T Ikawa, K Masuda, H Kawamoto and I Taniuchi
"Cascading suppression of transcriptional silencers by ThPOK seals helper T cell fate"
Nature Immonol., 9:1131-1121 (2008) - Wada H, Masuda K, Satoh R, Kakugawa K, Ikawa, T, Katsura Y, Kawamoto H:
"Adult T cell progenitors retain myeloid potential"
Nature, 452: 768-772 (2008) - Masuda K, Kakugawa K, Nakayama T, Minato M, Katsura Y, Kawamoto H:
"T cell lineage determination precedes the initiation of TCR gene rearrangement"
J. Immunol, 179, 3699-3706 (2007) - Masuda, K., H. Kubagawa, T. Ikawa, C. C. Chen, K. Kakugawa, M. Hattori, R. Kageyama, M. D. Cooper, N. Minato, Y. Katsura, and H. Kawamoto
"Prethymic T-cell development defined by the expression of paired immunoglobulin-like receptors"
EMBO J 24:4052-4060 (2005) - Ikawa, T., Kawamoto, H., and Murre, C.:
"Long term cultured E2A-deficient hematopoietic progenitor cells are pluripotent"
Immunity, 20, 349-360 (2004) - Kawamoto, H., Ikawa, T., Ohmura, K., Fujimoto, S., and Katsura, Y
"T cell progenitors emerge earlier than B cell progenitors in the murine fetal liver"
Immunity 12, 441-450 (2000)
Principal Investigator
- Hiroshi KAWAMOTO
- Team Leader
Members
- Kiyokazu KAKUGAWA
- Tomokatsu IKAWA
- Katrin Beate ISHII-SCHRADE
- Rumi SATO
- Riho WATANABE
- Asako SATO
- Yoshimoto KATSURA
- Visiting Scientist
- Nagahiro MINATO
- Visiting Scientist
- Haruka WADA
- Visiting Scientist
- Yuka KOBAYASHI
- Visiting Scientist
- Yoshihiro WATANABE
- Visiting Scientist
- Toshio KITAMURA
- Visiting Scientist