Chemical Genetics Laboratory
Research Areas
| Chemical Genetics is one of the new molecular genetics in which mutations in classical genetics are replaced by specific chemicals called bioprobes, and by which their unique cellular phenotypes are analyzed at the molecular level. This approach is providing more and more new insights into the molecular mechanisms underlying cellular response, cell cycle control and molecular recognition. In our laboratory, biological functions of a number of molecules targeted by bioprobes are studied by approaches using genomics, proteomics, and microbiology, in addition to chemical genetics. In particular, we focus on regulation of protein subcellular localization and protein post-translational modifications and their interplays such as acetylation, methylation ubiquitination and sumoylation. Our motto is to enjoy our research while aiming to create novel concepts in both basic and applied biologies. |
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Research Subject
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List of Selected Publications
| (1) | Dohi, Y., Ikura, T., Hoshikawa, Y., Katoh, Y., Ota, K., Nakanome, A., Muto, A., Omura, S., Ohta, T., Ito, A., Yoshida, M., Noda, T., and Igarashi, K.: "Bach1 inhibits oxidative stress-induced cellular senescence by impeding p53 function on chromatin." Nature Struct. Mol. Biol., 15: 1246-1254, (2008) |
| (2) | Kaida, D., Motoyoshi, H., Tashiro, E., Nojima, T., Hagiwara, M., Ishigami, K., Watanabe, H., Kitahara, T., Yoshida, T., Nakajima, H., Tani, T., Horinouchi, S., and Yoshida, M.: "Spliceostatin A targets SF3b and inhibits both splicing and nuclear retention of pre-mRNA." Nature Chem. Biol., 3: 576-583, (2007). |
| (3) | Shimazu, T., Horinouchi, S., and Yoshida, M.: "Multiple histone deacetylases and the CREB-binding protein regulate pre-mRNA 3'-end processing." J. Biol. Chem., 282: 4470-4478, (2007). |
| (4) | Matsuyama, A., Arai, R., Yashiroda, Y., Shirai, A., Kamata, A., Sekido, S., Kobayashi, Y., Hashimoto, A., Hamamoto, M., Hiraoka, Y., Horinouchi, S., and Yoshida, M.: "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Nature Biotechnol., 24: 841-847, (2006). |
| (5) | Shimazu, T., Komatsu, Y., Nakayama, K. I., Fukazawa, H., Horinouchi, S., and Yoshida, M.: "Regulation of SV40 large T antigen stability by reversible acetylation." Oncogene, 25: 7391-7400, (2006). |
| (6) | Kovacs, J. J., Murphy, P. J. M., Gaillard, S., Zhao, X., Wu, J.-T., Nicchitta, C. V., Yoshida, M., Toft, D. O., Pratt, W. B., and Yao, T.-P.: "HDAC6 regulates Hsp90 acetylation and chaperone-dependent activation of glucocorticoid receptor." Mol. Cell, 18: 601-607, (2005). |
| (7) | Kamura, T., Hara, T., Matsumoto, M., Ishida, N., Okumura, F., Hatakeyama, S., Yoshida, M., Nakayama, K., and Nakayama, K. I.: "Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27(Kip1) at G1 phase." Nature Cell Biol., 6: 1229-1235, (2004). |
| (8) | Yashiroda, Y. and Yoshida, M.: "Nucleo-cytoplasmic transport of proteins as a target for therapeutic drugs." Curr. Med. Chem., 10: 741-748, (2003). |
| (9) | Yoshida, Y., Chiba, T., Tokunaga, F., Kawasaki, H., Iwai, K., Suzuki, T., Ito, Y., Matsuoka, K., Yoshida, M., Tanaka, K., and Tai, T.: "E3 ubiquitin ligase that recognizes sugar chains." Nature, 418: 438-442, (2002). |
| (10) | Matsuyama, A., Shimazu, T., Sumida, Y., Saito, A., Yoshimatsu, Y., Seigneurin-Berny, D., Osada, H., Komatsu, Y., Nishino, N., Khochbin, S., Horinouchi, S., and Yoshida, M.: "In vivo destabilization of dynamic microtubules by HDAC6-mediated deacetylation." EMBO J., 21: 6820-6831, 2002. |