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RIKEN Center for Biosystems Dynamics Research Laboratory for Cell Field Structure

Team Leader: Atsuko Iwane (Ph.D.)

Research Summary

Atsuko  Iwane(Ph.D.)

Electron microscopy (EM) offers the best in resolution of extremely small biological specimens, providing images of the minutest of organelles and molecules that are responsible for phenomena fundamental to life including cell division, differentiation and proliferation. The resulting fine structural information has been extraordinary in helping our understanding of how morphology and function relate.
 We seek to image several target molecules and organelles, including the mitochondria and chloroplasts, and their surrounding environments inside a cell by using two different electron microscopy technologies. The first is Cryo-EM tomography, which produces a 3D image by scanning the field of view at multiple angles over an angular range from -70 degrees to 70 degrees relative to the perpendicular of the specimen plane used in transmission electron microscopy (TEM). The second is focused ion beam scanning electron microscopy (FIB-SEM), which uses SEM to scan a number of sections that are created by FIB. In this aim, we will combine our electron microscopy system with fluorescence microscopy to conduct dual imaging of the specimen in the same field of view. This will provide information on the dynamics of the specimen, which will complement the structure information and give us better understanding of the relationship between cell fate and cell morphology.

Main Research Fields

  • Biology

Related Research Fields

  • Biological Sciences

Keywords

  • electron microscope
  • 3D microstructure analysis
  • tomography

Selected Publications

  • 1.Fujii T, Iwane A.H., Yanagida T. Namba K.:
    "Direct visualization of secondary structures of F-actin by electron cryomicroscopy"
    Nature, 467, 724-728, 2010
  • 2.Nishikawa S., Arimoto I., Ikezaki K.,Sugawa M., Ueno H., Komori T., Iwane A.H., Yanagida T.:
    "Switch between large hand-over-hand and small inchworm-like steps in myosin VI"
    Cell, 142, 879-888, 2010
  • 3.Watanabe T.M., Yanagida T., Iwane A.H.:
    "Single molecular observation of self-regulated kinesin motility"
    Biochemistry, 49, 4654-4661, 2010
  • 4.Iwane A.H., Morimatsu M. Yanagida T.:
    "Recombinant α-actin for specific fluorescent labeling"
    Proceedings of the Japan Academy, Series B, Physical and Biological Sciences, 85, 491-499, 2009
  • 5.Iwaki M., Iwane A.H., Shimokawa T., Coock R., Yanagida T.:
    "Brownian search and catch mechanism for myosin-VI steps"
    Nature Chemical Biology, 5, 403-40, 2009
  • 6.Iwane A.H., Tanaka H., Morimoto S., Ishijima A., Yanagida T.
    "The neck domain of myosin II primarily regulates the actomyosin kinetics, not the stepsize"
    Journal of Molecular Biology, 353, 213-221, 2005
  • 7.Tanaka H., Homma K., Iwane A.H., Katayama E., Ikebe R., Yanagida T., Ikebe M.:
    "The motor domain determines the large step of myosin-V"
    Nature, 415, 192-195, 2002
  • 8.Kitamura K., Tokunaga M., Iwane A. H., Yanagida T.:
    "A single myosin head moves along an actin filament with regular steps of 5.3 nanometres"
    Nature, 397, 129-134, 1999
  • 9.Iwane A. H., Funatsu T., Harada Y., Tokunaga M., Yanagida T.
    "Single molecular assay of the individual ATP turnovers by a myosin-GFP fusion protein expressed in vitro"
    FEBS Letters, 407, 235-238, 1997
  • 10.Iwane A. H., Kitamura K., Tokunaga M., Yanagida T.:
    "Myosin subfragment-1 is fully equipped with factors essential for motor function"
    Biochemical and Biophysical Research Communications, 230, 76-80, 1997

Related Links

Lab Members

Principal investigator

Atsuko Iwane
Team Leader

Core members

Takako Ichinose
Technical Staff I
Rina Nagai
Technical Staff II
Takeshi Itabashi
Research Scientist

Contact Information

3-10-23, Kagamiyama,
Higashi-Hiroshima, Hiroshima
739-0046 JAPAN
Email: atsuko.iwane [at] riken.jp

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