RIKEN Center for Biosystems Dynamics Research Laboratory for Nano-Bio Probes

Team Leader: Takashi Jin (D.Sc.)

Japanese Page

Research Summary

Our research team develops novel nano-bio probes for visualization of molecular and cellular dynamics in biological systems. We will synthesize a variety of nano-bio-probes such as single-molecule imaging probes using quantum dots, cellular environment-sensitive probes, and cellular-state controllable probes using external magnetic fields. Furthermore, we will develop deep-tissue imaging probes to visualize cellular dynamics in vivo. We synthesize multi-colored fluorescent probes that emit NIR light (1000-1500 nm; the second optical window in living tissues), using cyanine dyes, fluorescent proteins, quantum dots, and rare-earth ions. By using the NIR fluorescent probes, cancer metastasis and immune reactions in vivo will be visualized at the cellular level. We are also developing probe delivery techniques using DDS for targeting cancer tissues and organs in small animals.

Keywords

Nano-Bio
Probe development
Quantum dots
Non-invasive imaging
near-infrared fluorescence

Selected Publications

1. Tsuboi S, Jin T.:
"Dual-colour (near-infrared/visible) emitting annexin V for fluorescence imaging of tumour cell apoptosis in vitro and in vivo"
RSC Adv. 38244-38250 (2020) doi: 10.1039/d0ra06495e.
2. Tiwari D.K, Tiwari M, Jin T.:
"Near-infrared fluorescent protein and bioluminescence-based probes for high-resolution in-vivo optical imaging"
Mater. Adv. 1, 967-987 (2020) doi:10.1039/d0ma00273a.
3. Tsuboi S, Jin T.:
"Shortwave-infrared (SWIR) fluorescence molecular imaging using indocyanine green-antibody conjugates for the optical diagnostics of cancerous tumours"
RSC Adv. 10. 28171–28179 (2020) doi: 10.1039/d0ra04710d.
4. Tsuboi S, Jin T.:
"BRET based dual-color (visible/near-infrared) molecular imaging using a quantum dot/EGFP-luciferase conjugate"
RSC Adv. 9, 34964-34971 (2019) doi: 10.1039/c9ra07011g.
5. Jin T.:
"Recent progress in NIR fluorophores emitting over 1000 nm for bioimaging"
ECS J. Solid State Sci.Technol. 8, R9-R13 (2019) doi: 10.1149/2.0111901jss.
6. Tsuboi S, Jin T.:
"Fluorescent recombinant protein conjugated near-infrared emitting quantum dots for in vitro and in vivo dual-color molecular imaging"
ChemBioChem 20, 565-575 (2019) https://doi.org/10.1002/cbic.201800506.
7. Tsuboi S, Jin T.:
"Recombinant Protein (Luciferase-IgG Binding Domain) Conjugated Quantum Dots for BRET-Coupled Near-Infrared Imaging of Epidermal Growth Factor Receptors"
Bioconjugate Chemistry 29. 1466-1574 (2018) doi:10.1021/acs.bioconjchem.8b00149.
8. Tsuboi S, Yamada S, Nakane Y, Sakata T, Yasuda H, and Jin T.:
"Water-soluble near-infrared fluorophores emitting over 1000 nm and their application to in vivo imaging in the second optical window (1000-1400nm)"
ECS J. Solid State Sci.Technol. 7. 3093-3101 (2018) doi: 10.1149/2.0141801jss.
9. Tsuboi S, Sasaki A, Sakata T, Yasuda H and Jin T.:
"Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging"
Chem. Comm. 53(68). 9450-9453 (2017) doi: 10.1039/c7cc04966h.
10. Tsuboi S and Jin T.:
"Bioluminescence Resonance Energy Transfer (BRET)-coupled Annexin V-functionalized Quantum Dots for Near-Infrared Optical Detection of Apoptotic Cells"
ChemBioChem 18(22). 2231-2235(2017) doi: 10.1002/cbic.201700486.

Lab Members

Principal investigator

Takashi Jin: Team Leader

Core members

Mahadeva Swamy M. M.: Research Scientist
Satoko Masa: Technical Staff I
Setsuko Tsuboi: Technical Staff I

Contact Information

Email: tjin [at] riken.jp