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RIKEN Center for Biosystems Dynamics Research Laboratory for Nano-Bio Probes

Team Leader: Takashi Jin (D.Sci.)

Research Summary

Takashi  Jin(D.Sci.)

Our research team develops novel nano-bio probes for visualization of molecular and cellular dynamics in biological systems. We will synthesize a variety of nano-bio-probes such as single-molecule imaging probes using quantum dots, cellular environment-sensitive probes, and cellular-state controllable probes using external magnetic fields. Furthermore, we will develop deep-tissue imaging probes to visualize cellular dynamics in vivo. We synthesize multi-colored fluorescent probes that emit NIR light (1000-1500 nm; the second optical window in living tissues), using cyanine dyes, fluorescent proteins, quantum dots, and rare-earth ions. By using the NIR fluorescent probes, cancer metastasis and immune reactions in vivo will be visualized at the cellular level. We are also developing probe delivery techniques using DDS for targeting cancer tissues and organs in small animals.


  • Nano-Bio
  • Probe development
  • Quantum dots
  • Non-invasive imaging
  • near-infrared fluorescence

Selected Publications

  • 1.Tsuboi S, Jin T.:
    "BRET based dual-color (visible/near-infrared) molecular imaging using a quantum dot/EGFP-luciferase conjugate"
    RSC Adv 9, 34964-34971 (2019) doi: 10.1039/c9ra07011g.
  • 2.Jin T.:
    "Recent progress in NIR fluorophores emitting over 1000 nm for bioimaging"
    ECS J. Solid State Sci.Technol. 8, R9-R13 (2019) doi: 10.1149/2.0111901jss.
  • 3.Tsuboi S, Jin T.:
    "Fluorescent recombinant protein conjugated near-infrared emitting quantum dots for in vitro and in vivo dual-color molecular imaging"
    ChemBioChem 20, 565-575 (2019) https://doi.org/10.1002/cbic.201800506.
  • 4.Komatsu N, Terai K, Imanishi A, Kamioka Y, Sumiyama K, Jin T, Okada Y, Nagai T, Matsuda M.:
    "A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging"
    Scientific Reports 8. 8984 (2018) doi: 10.1038/s41598-018-27174-x.
  • 5.Tsuboi S, Jin T.:
    "Recombinant Protein (Luciferase-IgG Binding Domain) Conjugated Quantum Dots for BRET-Coupled Near-Infrared Imaging of Epidermal Growth Factor Receptors"
    Bioconjugate Chemistry 29. 1466-1574 (2018) doi: 10.1021/acs.bioconjchem.8b00149.
  • 6.Tsuboi S, Yamada S, Nakane Y, Sakata T, Yasuda H, and Jin T.:
    "Water-soluble near-infrared fluorophores emitting over 1000 nm and their application to in vivo imaging in the second optical window (1000-1400nm)"
    ECS Journal of Solid State Science and Technology 7. 3093-3101 (2018) doi: 10.1149/2.0141801jss.
  • 7.Zamberlan F, Turyanska L, Patanè A, Liu Z, Williams HEL, Fay MW, Clarke PA, Imamura Y, Jin T, Bradshaw TD, Thomas NR and Grabowska AM.:
    "Stable DHLA-PEG capped PbS quantum dots: from synthesis to near-infrared biomedical imaging"
    Journal of Materials Chemistry B 6. 550-555(2018) doi: 10.1039/C7TB02912H
  • 8.Tsuboi S, Sasaki A, Sakata T, Yasuda H and Jin T.:
    "Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging"
    Chemical Communications 53(68). 9450-9453 (2017) doi: 10.1039/c7cc04966h.
  • 9.Tsuboi S and Jin T.:
    "Bioluminescence Resonance Energy Transfer (BRET)-coupled Annexin V-functionalized Quantum Dots for Near-Infrared Optical Detection of Apoptotic Cells"
    ChemBioChem 18(22). 2231-2235(2017) doi: 10.1002/cbic.201700486.
  • 10.Jin T, Tsuboi S, Komatsuzaki A, Imamura Y, Murakami Y, Sakata T and Yasuda H.:
    "Enhancement of aqueous stability and fluorescence brightness of indocyanine green using small calix[4]arene micelles for near-infrared fluorescence imaging"
    Medical Chemical Communications 7. 623-631(2016) doi: 10.1039/C5MD00580A.

Related Links

Lab Members

Principal investigator

Takashi Jin
Team Leader

Core members

Mahadeva Swamy M. M.
Research Scientist
Satoko Masa
Technical Staff I
Setsuko Tsuboi
Technical Staff II

Contact Information

Email: tjin [at] riken.jp