RIKEN Center for Biosystems Dynamics Research Laboratory for Organismal Patterning

Team Leader: Hiroshi Hamada (M.D., Ph.D.)

Japanese Page

Research Summary

My lab studies how left-right asymmetries develop in the mouse embryo. In particular, we focus on two types of cilia that are required for left-right symmetry breaking: rotating cilia that generate leftward fluid flow, and immotile cilia that sense the fluid flow. We also study the role of maternal and paternal epigenetic regulators in embryonic and post-natal development. We address these questions by integrating live imaging, structural biology, fluid dynamics and mathematical modeling.

Main Research Fields

Biological Sciences

Related Research Fields

Medicine, Dentistry & Pharmacy

Keywords

left-right asymmetry
embryogenesis
cilia
Epigenetic modification of germ cell genomes
Aging of germ cells

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

1.Takaoka, K., et al.:
"Both Nodal signaling and stochasticity select for prospective distal visceral endoderm in mouse embryos"
Nat Commun. Accepted.
2.Minegishi K., et al.:
"A Wnt5 activity asymmetry and intercellular signaling polarize node cells for breaking left-right symmetry in the mouse embryo"
Dev. Cell 40:439-452 (2017)
3.*Shinohara K., et al.:
"Absence of radial spokes in mouse node cilia is required for rotational movement but confers ultrastructural instability as a trade-off."
Dev Cell 35.236-246 (2015)
4.*Dong, F., et al.:
"Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm."
J. Cell. Biol. 204:203-213 (2014)
5.*Takamatsu, A., et al.:
"Hydrodynamic phase synchronization in mouse node cilia"
Physical Review Letters.110:248107 (2014)
6.*Nakamura T., et al.:
"Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA in the mouse embryo."
Nat Commun. 3.1322 (2012)
7.*Yoshiba S., et al.:
"Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2."
Science 338.226-231 (2012)
8.*Shinohara K., et al.:
"Two rotating cilia in the node cavity are sufficient to break left-right symmetry in the mouse embryo."
Nat Commun. 3.622 (2012)
9.*Takaoka K., et al.:
"Origin and role of distal visceral endoderm, a group of cells that determines anterior-posterior polarity of the mouse embryo."
Nat Cell Biol 13. 743-752 (2011)
10.*Hashimoto M., et al.:
"Planar polarization of the node cells determines the rotation axis of the node cilia."
Nat Cell Biol 12.170-176 (2010)