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RIKEN BioResource Research Center Bioresource Engineering Division

Head: Atsuo Ogura (D.V.M., Ph.D.)

Research Summary

Atsuo  Ogura(D.V.M., Ph.D.)

To sustain the reliability of the Bioresource Project, it is critically important to continuously maintain and distribute bioresources at a high quality. We develop new techniques for this purpose. The major subjects include those essential for the projects of mouse and cell resources, such as nuclear transfer, assisted fertilization (microinjection), establishment of stem cell lines of various origins, and cryopreservation of embryos/gametes. We also undertake studies on developmental/reproductive biology and epigenetics by using the techniques we develop.

Main Research Fields

  • Biological Sciences

Related Research Fields

  • Biology
  • Agricultural Sciences
  • Medicine, Dentistry & Pharmacy

Keywords

  • Fertilization
  • Genome Reprogramming
  • Germ Cells
  • Epigenetics

Selected Publications

  • 1.Inoue, K., Hirose, M., Inoue, H., Hatanaka, Y., Honda, A., Hasegawa, A., Mochida, K., Ogura, A.:
    "The rodent-specific microRNA cluster within the Sfmbt2 gene is imprinted and essential for placental development."
    Cell Rep 19, 949-956 (2017).
  • 2.Kaminuma, O., Katayama, K., Inoue, K., Saeki, M., Nishimura, T., Kitamura, N., Shimo, Y., Tofukuji, S., Ishida, S., Ogonuki, N., Kamimura, S., Oikawa, M., Katoh, S., Mori, A., Shichijo, M., Hiroi, T., Ogura, A.:
    "Hyper-reactive cloned mice generated by direct nuclear transfer of antigen-specific CD4+ T cells."
    EMBO Rep 18, 885-893 (2017)
  • 3.Hatanaka, Y., Inoue, K., Oikawa, M., Kamimura, S., Ogonuki, N., Kodama, E., Ohkawa, Y., Tsukada, Y., Ogura, A.:
    "Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposon."
    Proc Natl Acad Sci USA 112, 14641-14646, (2015)
  • 4.Oikawa, M., Inoue, K., Shiura, H., Matoba, S., Kamimura, S., Hirose, M., Mekada, K., Yoshiki, A., Tanaka, A., Abe, K., Ishino, F., and Ogura, A.:
    "Understanding the X chromosome inactivation cycle in mice: A comprehensive view provided by nuclear transfer."
    Epigenetics 9, 204–211 (2014).
  • 5.Ogura, A., Inoue, K., and Wakayama, T.:
    "Recent advancements in cloning by somatic cell nuclear transfer."
    Philos Trans R Soc Lond B Biol Sci 368, 20110329 (2013).
  • 6.Morimoto, H., Iwata, K., Ogonuki, N., Inoue, K., Ogura, A., Kanatsu-Shinohara, M., Morimoto, T., Yabe-Nishimura, C., and Shinohara, T.:
    "ROS are required for mouse spermatogonial stem cell self-renewal."
    Cell Stem Cell 12, 774–786 (2013).
  • 7.Matoba, S., Inoue, K., Kohda, K., Sugimioto, M., Mizutani, E., Ogonuki, N., Nakamura, T., Abe, K., Nakano, T., Ishino, F., and Ogura, A.:
    "RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos. "Proc Natl Acad Sci USA 108, 20621-20626 (2011).
  • 8.Inoue, K., Kohda, T., Sugimoto, M., Sado, T., Ogonuki, N., Matoba, S., Shiura, H., Ikeda, R., Mochida, K., Fujii, T., Sawai, K., Otte, AP., Tian, XC., Yang, X., Ishino, F., Abe, K., and Ogura, A.:
    "Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer."
    Science 330 496-499 (2010).
  • 9.Honda, A., Hirose, M., Hara, K., Matoba, S., Inoue, K., Miki, H., Hiura, H., Kanatsu-Shinohara, M., Kanai, Y., Kono, T., Shinohara, T., and Ogura, A.:
    "Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells."
    Proc Natl Acad Sci USA 104, 12389-12394 (2007).
  • 10.Ogonuki, N., Mochida, K., Miki, H., Inoue, K., Fray, M., Iwaki, T., Moriwaki, K., Obata, Y., Morozumi, K., Yanagimachi, R., and Ogura, A.:"Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring"Proc Natl Acad Sci USA 103, 13098-13103 (2006).

Recent Research Results

Related Links

Lab Members

Principal investigator

Atsuo Ogura
Head

Core members

Kimiko Inoue
Senior Research Scientist
Shogo Matoba
Senior Research Scientist
Keiji Mochida
Senior Technical Scientist
Narumi Ogonuki
Senior Technical Scientist
Masashi Hada
Visiting Researcher
Kento Miura
Postdoctoral Researcher
Toshiko Tomishima
Technical Staff II
Michiko Hirose
Technical Staff II
Ayumi Hasegawa
Technical Staff II

Contact Information

3-1-1 Koyadai
Tsukuba, Ibaraki
305-0074 Japan

Email: ogura [at] rtc.riken.jp

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