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RIKEN Baton Zone Program Advanced Electron Microscope Development Unit

Unit Leader: Koji Yonekura (Ph.D.)

Research Summary

Koji  Yonekura(Ph.D.)

We address R & D of the advanced cryo-electron microscopy through combining the knowledge of JEOL on the electron microscope with the elemental technologies fostered by operation and development of SPring-8/SACLA.

Main Research Fields

  • Biological Sciences

Related Research Fields

  • Chemistry
  • Biology
  • Advanced cryo-electron microscopy
  • High-resolution single particle cryo-EM
  • Electron 3D crystallography (3D ED / microED)

Selected Publications

  • 1. Inoue S., Nikaido K., Higashino T., Arai S., Tanaka M., Kumai R., Tsuzuki S., Horiuchi S., Sugiyama H., Segawa Y., Takaba K., Maki-Yonekura S., Yonekura K., Hasegawa T.:
    "Emerging disordered layered-herringbone phase in organic semiconductors unveiled by electron crystallography"
    Chem. Mater. 34, 72–83 (2022)
  • 2. Yonekura K., Maki-Yonekura S., Naitow H., Hamaguchi T., Takaba K.:
    "Machine learning-based real-time object locator / evaluator for cryo-EM data collection"
    Commun. Biol. 4, 1044 (2021)
  • 3. Hamaguchi T., Kawakami K., Shinzawa-Itoh K., Inoue-Kashino N., Itoh S., Ifuku K., Yamashita E., Maeda K., Yonekura K., Kashino Y.:
    "Structure of the far-red light utilizing photosystem I of Acaryochloris"
    Nat. Commun. 12, 2333 (2021).
  • 4. Kato K., Takaba K., Maki-Yonekura S., Mitoma N., Nakanishi Y., Nishihara T., Hatakeyama T., Kawada T., Hijikata Y., Pirillo J., Scott L.T., Yonekura K., Segawa Y., Itami K.:
    "Double-helix supramolecular nanofibers assembled from negatively curved nanographenes."
    J. Am. Chem. Soc. 143, 5465–5469 (2021).
  • 5. Kato K., Miyazaki N., Hamaguchi T., Nakajima Y., Akita F., Yonekura K., Shen J.-R.:
    "High-resolution cryo-EM structure of photosystem II: Effects of electron beam damage."
    Commun. Biol. 4, 382 (2021).
  • 6. Maki-Yonekura S., Hamaguchi T., Naitow H., Takaba K., Yonekura K.:
    "Advances in cryo-EM and ED with a cold-field emission beam and energy filtration: - refinements of the CRYO ARM 300 system in RIKEN SPring-8 Center –."
    Microscopy 70, 232–240 (2021)
  • 7. Takaba K., Maki-Yonekura S., Inoue S., Hasegawa T., Yonekura K.:
    "Protein and organic-molecular crystallography with 300kV electrons on a direct electron detector"
    Front. Mol. Biosci. 7, 612226 (2021)
  • 8. Naitow, H., Hamaguchi, T., Maki-Yonekura, S., Isogai, M., Yoshikawa, N. and Yonekura, K.:
    "Apple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome release"
    Commun. Biol. 3: 488 (2020).
  • 9. Hamaguchi T., Maki-Yonekura S., Naitow H., Matsuura Y., Ishikawa T. and Yonekura K.:
    "A new cryo-EM system for single particle analysis"
    J. Structl. Biol. 207: 40-48 (2019).
  • 10. Yonekura K., Ishikawa T. and Maki-Yonekura S.:
    "A new cryo-EM system for electron 3D crystallography"
    J. Structl. Biol. 206: 243-253 (2019).

Lab Members

Principal investigator

Koji Yonekura
Unit Leader

Contact Information

1-1-1 Kouto, Sayo-cho Sayo-gun, Hyogo 679-5148 Japan
Email: yone [at] spring8.or.jp

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