RIKEN Center for Sustainable Resource Science Drug Discovery Seed Compounds Exploratory Unit
Unit Leader: Minoru Yoshida (D.Agr.)
Research Summary

The mission of the Seed Compounds Exploratory Unit is to contribute to searching for active compound candidates (hit compounds) for drug targets through high throughput screening (HTS) from compound libraries and, further, to identifying hit compounds (true hits) that truly act on the targets.
Main Research Fields
- Chemistry
Related Research Fields
- Biology
Keywords
- Chemical Biology
- Epigenetics
- Mode of Action
- Transcriptional Regulation
- Chemical Screening for Drug Discovery
Selected Publications
- 1.Matsumoto, K., Kose, S., Kuwahara, I., Yoshimura, M., Imamoto, N., Yoshida, M.:
"Y-box protein-associated acidic protein (YBAP1/C1QBP) affects the localization and cytoplasmic functions of YB-1."
Sci. Rep., 8, 6198 (2018) - 2.Takase, S., Kurokawa, R., Arai, D., Kanemoto Kanto, K,, Okino, T., Nakao, Y., Kushiro, T., Yoshida, M., Matsumoto, K.:
"A quantitative shRNA screen identifies ATP1A1 as a gene that regulates cytotoxicity by aurilide B."
Sci. Rep., 7, 2002 (2017) - 3.Piotrowski, J.S., Li, S.C., Deshpande, R., Simpkins, S.W., Nelson, J., Yashiroda, Y., Barber, J.M., Safizadeh, H., Wilson, E., Okada, H., Gebre, A.A., Kubo, K., Torres, N., Leblanc, M.B., Andrusiak, K., Okamoto, R., Yoshimura, M., van Leeuwen, J., Shirahige, K., Baryshnikova, A., Brown, G., Saito, T., Costanzo, M., Andrews, B., Ohya, Y., Osada, H., Yoshida, M., Myers, C.L., Boone, C.:
"Functional annotation of chemical libraries across diverse biological processes."
Nat. Chem. Biol., 13, 982-993 (2017) - 4.Nakaoka, S., Sasaki, K., Ito, A., Nakao, Y., Yoshida, M.:
"A genetically encoded FRET probe to detect intranucleosomal histone H3K9 or H3K14 acetylation using BRD4, a BET family member."
ACS Chem. Biol., 11, 729-33 (2016) - 5.Sun, X., Hirai, G., Ueki, M., Hirota, H., Wang, Q., Hongo, Y., Nakamura, T., Hitora, Y., Takahashi, H., Sodeoka, M., Osada, H., Hamamoto, M., Yoshida, M., Yashiroda, Y.:
"Identification of novel secreted fatty acids that regulate nitrogen catabolite repression in fission yeast."
Sci. Rep., 6, 20856 (2016) - 6.Ling, F., Niu, R., Hatakeyama, R., Goto, Y., Shibata, T., and Yoshida, M.:
"ROS stimulate mitochondrial allele segregation towards homoplasmy in human cells."
Mol. Biol. Cell, 27, 1684-93 (2016) - 7.Ito, A., Shimazu, T., Maeda, S., Shah, A.A., Tsunoda, T., Iemura, S., Natsume, T., Suzuki, T., Motohashi, H., Yamamoto, M., Yoshida, M.:
"The subcellular localization and activity of cortactin is regulated by acetylation and interaction with Keap1."
Sci. Signal., 8, ra120 (2015) - 8.Arita, Y., Nishimura, S., Ishitsuka, R., Kishimoto, T., Ikenouchi, J., Ishii, K., Umeda, M., Matsunaga, S., Kobayashi, T., Yoshida, M.:
"Targeting cholesterol in a liquid-disordered environment by theonellamides modulates cell membrane order and cell shape."
Chem. Biol., 222, 604-610 (2015) - 9.Feldman, J.L., Dittenhafer-Reed, K.E., Kudo, N., Thelen, J.N., Ito, A., Yoshida, M., Denu, J.M.:
"Kinetic and structural basis for acyl-group selectivity and NAD(+) dependence in sirtuin-catalyzed deacylation."
Biochemistry, 54, 3037-3050 (2015) - 10.Hirohama, M., Kumar, A., Fukuda, I., Matsuoka, S., Igarashi, Y., Saitoh, H., Takagi, M., Shin-Ya, K., Honda, K., Kondoh, Y., Saito, T., Nakao, Y., Osada, H., Zhang, K.Y., Yoshida, M., Ito, A.:
"Spectomycin B1 as a novel SUMOylation inhibitor that directly binds to SUMO E2."
ACS Chem. Biol., 8, 2635-2642 (2013)
Related Links
Lab Members
Principal investigator
- Minoru Yoshida
- Unit Leader
Core members
- Akiko Idei
- Deputy Unit Leader
- Ken Matsumoto
- Senior Research Scientist
- Kenji Ogawa
- Senior Visiting Scientist
- Hiroki Kobayashi
- Visiting Scientist
- Mie Kubota
- Visiting Scientist
- Seiji Matsuoka
- Technical Scientist
- Kousiki Mino
- Senior Technical Staff
- Shin-ya Okamoto
- Senior Technical Staff
- Satoko Maeda
- Technical Staff I
- Iku Kuwahara
- Technical Staff I
- Michiru Iwashita
- Technical Staff I
- Haruna Nishimura
- Technical Staff I
- Mari Agawa
- Technical Staff I
- Takeshi Sonoda
- Technical Staff I
- Yasue Ichikawa
- Technical Staff I
- Akiko Nakata
- Technical Staff II
- Taeko Wakana
- Technical Staff II
- Toshie Kaizuka
- Technical Staff II
- Hirochika Kawakami
- Research Fellow