RIKEN Hakubi Research Teams Human Biomimetic System RIKEN Hakubi Research Team

RIKEN Hakubi Team Leader: Masaya Hagiwara (Ph.D.)

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Research Summary

The overall objective is the development of in-vitro experimental platform to reconstitute the biological system outside our bodies by controlling cellular micro-environments. Especially lung airway is the one of my major targets. The scientific question is how the airway cells can organize such a sophisticated structure by themselves. The repetitive feedbacks between in-vitro experiments and mathematical model give us a tremendous amount of useful information, and I am challenging to unveil the system underlying self-organization of tissue formation by directing complex tissue structure. Development of Organ or Body-on-a-Chip system is another research target for the animal testing alternative.

Main Research Fields

Complex Systems

Related Research Fields

Informatics
Interdisciplinary Science & Engineering
Mathematical & Physical Sciences
Chemistry
Engineering
Biological Sciences
Biology
Medicine, Dentistry & Pharmacy
Biomedical engineering/Biomaterial science and engineering
Nano/Microsystems
Intelligent mechanics/Mechanical systems

Keywords

In vitro tissue formation
Tissue imaging
Organ on a Chip
Culture Control

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

1. Takano, A., Koh, I., and Hagiwara, M.:
"3D Culture Platform for Enabling Large-Scale Imaging and Control of Cell Distribution into Complex Shapes by Combining 3D Printing with a Cube Device"
Micromachines, 13, 156, (2022)
2. Hagiwara, M., Maruyama, H., Akiyama, M., Koh, I., Arai, F.:
"Weakening of resistance force by cell–ECM interactions regulate cell migration directionality and pattern formation"
Communications Biology volume 4, 808 (2021)
3. Hagiwara, M., Koh, I.:
"Engineering approaches to control and design the in vitro environment towards the reconstruction of organs"
Development, Growth & Differentiation, 62, pp158-166 (2020)
4. *Hagiwara, M., Nobata, R., Kawahara, T.;
"Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations"
Journal of Visualized Experiments, 145, e5924, (2019)
5. *Hagiwara, M., Nakase, I.:
"Epidermal growth factor induced macropinocytosis directs branch formation of lung epithelial cells"
Biochemical and Biophysical Research Communications, 507, 297-303, (2018).
6. *Hagiwara, M., Nobata, R., Kawahara, T.:
"High repeatability from 3D experimental platform for quantitative analysis of cellular branch pattern formations"
Integrative Biology, 10, 306-312, (2018).
7. *Hagiwara, M., Nobata, R., Kawahara, T.:
"Large scale imaging by fine spatial alignment of multi-scanning data with gel cube device"
Applied Sciences, 8, doi:10.3390/app8020235, (2018).
8. *Hagiwara, M., Maruta, M., Marumoto, M.:
"In Vitro Experimental Model for the Long-Term Analysis of Cellular Dynamics During Bronchial Tree Development from Lung Epithelial Cells"
Tissue Engineering Part C: Methods, 23, 323-332, (2017).
9. *Hagiwara, M:
"An in vitro–in silico interface platform for spatiotemporal analysis of pattern formation in collective epithelial cells"
Integrative Biology, 8, 861-868, (2016).
10. *Hagiwara, M., Nobata, R., Kawahara, T.:
"Tissue in Cube: In Vitro 3D Culturing Platform with Hybrid Gel Cubes for Multidirectional Observations"
Advanced Healthcare Materials, 5, 1566-1571, (2016).

Recent Research Results

Apr. 11, 2023
Simple but revolutionary modular organoids

Annual research report

Lab Members

Principal investigator

Masaya Hagiwara: RIKEN Hakubi Team Leader

Core members

Raja Kumar Vadivelu: Special Postdoctoral Researcher
Isabel Siew Yin Koh: Postdoctoral Researcher
Yen Xuan Ngo: Postdoctoral Researcher
Satoko Kinoshita: Assistant

Careers

Position	Deadline
Seeking a Research Scientist or Postdoctoral Researcher (K24037)	Open until filled

Contact Information

Bioscience Bldg.
2-1 Hirosawa,
Wako, Saitama,
351-0198, Japan
Tel：+81-(0)48-467-3630
Email: masaya.hagiwara [at] riken.jp