Research Summary

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

• 1. Oguchi, Y., Ozaki, Y., Abdelmoez, M.N. and Shintaku, H.:
  "NanoSINC-seq dissects the isoform diversity in subcellular compartments of single cells"
  Science Advances, Vol. 7, No.15 (2021), eabe0317.
• 2. Subramanian Parimalam, S., Abdelmoez, M.N., Tsuchida, A., Sotta, N., Tanaka, M., Kuromori, T., Fujiwara, T., Hirai, M.Y., Yokokawa, E., Oguchi, Y. and Shintaku, H.:
  "Targeted permeabilization of cell wall and extraction of charged molecules from single cells in intact plant clusters using a focused electric field"
  Analyst, Vol. 146 (2021), pp. 1604-1611.
• 3. Abdelmoez M.N., Oguchi, Y.,Ozaki, Y., Yokokawa, R., Kotera, H., and Shintaku, H.:
  "Distinct Kinetics in Electrophoretic Extraction of Cytoplasmic RNA from Single Cells"
  Analytical Chemistry, Vol. 92, No. 1(2020), pp. 1485-1492.
• 4. Abdelmoez, M.N.^*, Iida, K.^*, Oguchi, Y.^*, Nishikii, H., Yokokawa, R., Kotera, H., Uemura, S., Santiago, J.G. and Shintaku, H.:
  "SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology"
  Genome Biology, (2018) DOI:. 10.1186/s13059-018-1446-9.
• 5. Subramanian Parimalam, S., Oguchi, Y., Abdelmoez, M.N., Tsuchida, A., Ozaki, Y., Yokokawa, R., Kotera, H., and Shintaku, H.:
  "Electrical Lysis and RNA Extraction from Single Cells Fixed by Dithio-bis(succinimidyl propionate)"
  Analytical Chemistry, Vol. 90, No. 21 (2018), pp.12512-12518.
• 6. Khnouf, R., Shore, S., Han, C.M., Henderson, J.M., Munro, S.A., McCaffrey, A.P., Shintaku, H., and Santiago, J.G.:
  "Efficient Production of On-target Reads for Small RNA Sequencing of Single Cells Using Modified Adapters"
  Analytical Chemistry, Vol. 90, No. 21 (2018), pp.12609-12615.
• 7. *Kuriyama, K.^*, Shintaku, H.^*, and Santiago, J.G.:
  "Protocol for microfluidic system to automate the preparation and fractionation of the nucleic acids in the cytoplasm versus nuclei of single cells"
  Bio-protocol, Vol. 6, Issue 12, (2016), e1844.
• 8. *Kuriyama, K.*, Shintaku, H.*, and Santiago, J.G.:
  "Isotachophoresis for Fractionation and Recovery of Cytoplasmic RNA and Nucleus from Single Cells"
  Electrophoresis, Vol. 36, Issue 14(2015) pp. 1658-1662.
• 9. *Shintaku, H., Palko, J.W., Sanders, G.M., and Santiago, J.G.:
  "Increasing Hybridization Rate and Sensitivity of Bead Based Assays Using Isotachophoresis"
  Angewandte Chemie International Edition,Vol. 53 (2014), pp. 13813-13816.
• 10. *Shintaku, H., Nishikii, H., Marshall, L.A., Kotera, H., and Santiago, J.G.:
  "On-Chip Separation and Analysis of RNA and DNA from Single Cells"
  Analytical Chemistry, Vol. 86, No. 4 (2014), pp 1953–1957.

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