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RIKEN Center for Integrative Medical Sciences Nucleic Acid Diagnostic System Development Unit

Unit Leader: Hideya Kawaji (Ph.D.)

Research Summary

Kengo Usui (Ph.D.)

Based on “SmartAmp method” and “Eprobe” developed by Genetic Diagnostic Technology Unit at our center, we are aiming to apply to genetic diagnostic technology that can be used in medical field. We promote clinical research using our technology by collaborating with actual medical institutions and hospitals, and evaluate and improve as a genetic diagnosis kit. Moreover, in order to realize genetic diagnosis technology as an in-vitro diagnostics, we are preparing data necessary for deriving technology to companies.

Main Research Fields

  • Biological Sciences

Related Research Fields

  • Medicine, Dentistry & Pharmacy


  • Genetic Diagnosis
  • Nucleic acid amplification
  • Infectious diseases detecting technology
  • SNP genotyping
  • Somatic mutation detection

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

  • 1.*Takase, Y., Usui, K., Shimizu, K., Kimura, Y., Ichihara, T., Ohkawa, T., Atsumi, J., Enokida, Y., Nakazawa, S., Obayashi, K., Ohtaki, Y., Nagashima, T., Mitani, Y., and Takeyoshi, I.:
    "Highly sensitive detection of a HER2 12-base pair duplicated insertion mutation in lung cancer using the Eprobe-PCR method."
    PLoS One. 12, e0171225 (2017).
  • 2.*Enokida, Y., Shimizu, K., Atsumi, J., Kakegawa, S., Takase, Y., Kaira, K., Yashima, H., Araki, T., Nakazawa, S., Ohtaki, Y., Nagashima, T., Alexander, L., Usui, K., Ishikawa, T., Hayashizaki, Y., and Takeyoshi, I.:
    "Prognostic potential of the MDM2 309T>G polymorphism in stage I lung adenocarcinoma."
    Cancer Med. 5, 1791-801 (2016).
  • 3.*Kimura, Y., Soma, T., Kasahara, N., Delobel, D., Hanami, T., Tanaka, Y., de Hoon, MJ., Hayashizaki, Y., Usui, K., and Harbers, M.:
    "Edesign: Primer and Enhanced Internal Probe Design Tool for Quantitative PCR Experiments and Genotyping Assays."
    PLoS One. 11, e0146950 (2016).
  • 4.*Atsumi, J., Hanami, T., Enokida, Y., Ogawa, H., Delobel, D., Mitani, Y., Kimura, Y., Soma, T., Tagami, M., Takase, Y., Ichihara, T., Takeyoshi, I., Usui, K., Hayashizaki, Y., and Shimizu K.:
    "Eprobe-mediated screening system for somatic mutations in the KRAS locus."
    Oncol Rep. 33, 2719-2727 (2015).
  • 5.*Enokida, Y., Shimizu, K., Kakegawa, S., Atsumi, J., Takase, Y., Miyamae, Y., Nagashima, T., Ohtaki, Y., Kaira, K., Sunaga, N., Yanagitani, N., Yoshino, R., Tsunekawa, K., Igai, H., Kamiyoshihara, M., Usui, K., Lezhava, A., Tomizawa, Y., Ishikawa, T., Murakami, M., Hayashizaki, Y., and Takeyoshi I.:
    "Single-nucleotide polymorphism (c.309T>G) in the MDM2 gene and lung cancer risk."
    Biomed Rep. 2, 719-724 (2014).
  • 6.*Hanami, T., Delobel, D., Kanamori, H., Tanaka, Y., Kimura, Y., Nakasone, A., Soma, T., Hayashizaki, Y., Usui, K., and Harbers, M.:
    "Eprobe mediated real-time PCR monitoring and melting curve analysis."
    PLoS One. 8, e70942 (2013).
  • 7.*Enokida, Y., Shimizu, K., Atsumi, J., Lezhava, A., Tanaka, Y., Kimura, Y,, Soma, T., Hanami, T., Kawai, Y., Usui, K., Okano, Y., Kakegawa, S., Ogawa, H., Miyamae, Y., Miyagi, Y., Nakayama, H., Ishikawa, T., Hayashizaki, Y., and Takeyoshi I.:
    "Rapid detection of SNP (c.309T>G) in the MDM2 gene by the Duplex SmartAmp method."
    PLoS One. 8, e60151 (2013).
  • 8.*Kimura, Y., Hanami, T., Tanaka, Y., De Hoon, MJ., Soma, T., Harbers, M., Lezhava, A., Hayashizaki, Y., and Usui, K.:
    "Effect of thiazole orage double-labeled thymidine on DNA duplex formation."
    Biochemistry, 51, 6056-6067 (2012).
  • 9.*Kawai, Y., Kimura, Y., Lezhava, A., Kanamori, H., Usui, K., Hanami, T., Soma, T., Morlighem, JÉ., Saga, S., Ishizu, Y., Aoki, S., Endo, R., Oguchi-Katayama, A., Kogo, Y., Mitani, Y., Ishidao, T., Kawakami, C., Kurata, H., Furuya, Y., Saito, T., Okazaki, N., Chikahira, M., Hayashi, E., Tsuruoka, S., Toguchi, T., Saito, Y., Ban, T., Izumi, S., Uryu, H., Kudo, K., Sakai-Tagawa, Y., Kawaoka, Y., Hirai, A., Hayashizaki, Y., and Ishikawa, T.:
    "One-step detection of the 2009 pandemic influenza A(H1N1) virus by the RT-SmartAmp assay and its clinical validation."
    PLoS One, 7, e30236 (2012).
  • 10.*Tanaka, Y., Kimura, Y., Mitani, Y., Kawai, Y., Lezhava, A., Noma, S., Tagami, M., Kawai, J., Hayashizaki, Y., and Usui, K.:
    "Effects of the turn-back primer on intermediate product generation in isothermal DNA amplification."
    Biotechniques. 49, 888-892 (2010).

Related Links

Lab Members

Principal investigator

Hideya Kawaji
Unit Leader

Core members

Yuji Tanaka
Research Scientist

Contact Information

1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama City, Kanagawa,
230-0045, Japan
Tel: +81-(0)45-503-9222
Fax: +81-(0)45-503-9216
Email: kawaji [at] gsc.riken.jp