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Medical Sciences Innovation Hub Program Biomarker Analysis Group

Group Director: Osamu Ohara (Ph.D.)

Research Summary

Osamu  Ohara(Ph.D.)

A mission of our research group is to develop and operate pipelines in biomarker exploration based on advanced genomics technologies, which shows remarkable progress in recent years. The target biomarkers cover various biomolecules such as protein, RNA and DNA, and we design and manage the measurement pipelines from single-cell resolution to the organ/tissue hierarchy with an expected comprehensiveness, depending on purpose of each project. In addition, the evaluation of reproducibility of these measurements and the quality control are also our important missions. We will conduct research and development with the view of not only data measurement but also primary data analysis.

Main Research Fields

  • Biology

Related Research Fields

  • Medicine, Dentistry & Pharmacy
  • Biophysics
  • Molecular biology

Keywords

  • Molecular biology
  • biomarker analysis

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

  • 1.Shirasaki Y, Ohara O.:
    "Challenges in Developing Protein Secretion Assays at a Single-Cell Level."
    Methods Mol Biol. 2018;1808:1-7. doi:10.1007/978-1-4939-8567-8_1. PubMed PMID: 29956169.
  • 2.*Fujiki R, Ikeda M, Yoshida A, Akiko M, Yao Y, Nishimura M, Matsushita K, Ichikawa T, Tanaka T, Morisaki H, Morisaki T, Ohara O.:
    "Assessing the Accuracy of Variant Detection in Cost-Effective Gene Panel Testing by Next-Generation Sequencing. J Mol Diagn. 2018 Jun 25. pii: S1525-1578(17)30554-8."
    doi:10.1016/j.jmoldx.2018.04.004. [Epub ahead of print] PubMed PMID: 29953964.
  • 3.Isobe Y, Kawashima Y, Ishihara T, Watanabe K, Ohara O, Arita M.:
    "Identification of Protein Targets of 12/15-Lipoxygenase-Derived Lipid Electrophiles in Mouse Peritoneal Macrophages Using Omega-Alkynyl Fatty Acid."
    ACS Chem Biol. 2018 Apr 20;13(4):887-893. doi: 10.1021/acschembio.7b01092. Epub 2018 Mar 1. PubMed PMID: 29461797.
  • 4.Kadowaki T, Ohnishi H, Kawamoto N, Hori T, Nishimura K, Kobayashi C, Shigemura T, Ogata S, Inoue Y, Kawai T, Hiejima E, Takagi M, Imai K, Nishikomori R, Ito S, Heike T, Ohara O, Morio T, Fukao T, Kanegane H.:
    "Haploinsufficiency of A20 causes autoinflammatory and autoimmune disorders. J Allergy Clin Immunol. 2018 Apr;141(4):1485-1488.e11."
    doi: 10.1016/j.jaci.2017.10.039. Epub 2017 Dec 11.PubMed PMID: 29241730.
  • 5.Bin BH, Bhin J, Takaishi M, Toyoshima KE, Kawamata S, Ito K, Hara T, Watanabe T, Irié T, Takagishi T, Lee SH, Jung HS, Rho S, Seo J, Choi DH, Hwang D, Koseki H, Ohara O, Sano S, Tsuji T, Mishima K, Fukada T.:
    "Requirement of zinc transporter ZIP10 for epidermal development: Implication of the ZIP10-p63 axis in epithelial homeostasis. Proc Natl Acad Sci U S A. 2017 Nov 14;114(46):12243-12248. "
    doi:10.1073/pnas.1710726114. Epub 2017 Oct 23. PubMed PMID: 29078349; PubMed Central PMCID: PMC5699059.
  • 6.Dong Y, Isono KI, Ohbo K, Endo TA, Ohara O, Maekawa M, Toyama Y, Ito C, Toshimori K, Helin K, Ogonuki N, Inoue K, Ogura A, Yamagata K, Kitabayashi I, Koseki H.:
    "EPC1/TIP60-Mediated Histone Acetylation Facilitates Spermiogenesis inMice."
    Mol Cell Biol. 2017 Sep 12;37(19). pii: e00082-17. doi:10.1128/MCB.00082-17. Print 2017 Oct 1. PubMed PMID: 28694333; PubMed Central PMCID: PMC5599718.
  • 7.Endoh M, Endo TA, Shinga J, Hayashi K, Farcas A, Ma KW, Ito S, Sharif J, Endoh T, Onaga N, Nakayama M, Ishikura T, Masui O, Kessler BM, Suda T, Ohara O, Okuda A, Klose R, Koseki H.:
    "PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes."
    Elife. 2017 Mar 17;6. pii: e21064. doi: 10.7554/eLife.21064. Erratum in: Elife. 2017 Apr 25;6:. PubMed PMID: 28304275; PubMed Central PMCID: PMC5375644.
  • 8.*Fujiki R, Hijikata A, Shirai T, Okada S, Kobayashi M, Ohara O.:
    "Molecular mechanism and structural basis of gain-of-function of STAT1 caused by pathogenic R274Q mutation."
    J Biol Chem. 2017 Apr 14;292(15):6240-6254. doi:10.1074/jbc.M116.753848. Epub 2017 Mar 3. PubMed PMID: 28258222; PubMed Central PMCID: PMC5391754.
  • 9.Kagawa R, Fujiki R, Tsumura M, Sakata S, Nishimura S, Itan Y, Kong XF, Kato Z, Ohnishi H, Hirata O, Saito S, Ikeda M, El Baghdadi J, Bousfiha A, Fujiwara K, Oleastro M, Yancoski J, Perez L, Danielian S, Ailal F, Takada H, Hara T, Puel A, Boisson-Dupuis S, Bustamante J, Casanova JL, Ohara O, Okada S, Kobayashi M.:
    "Alanine-scanning mutagenesis of human signal transducer and activator of transcription 1 to estimate loss- or gain-of-function variants."
    J Allergy Clin Immunol. 2017 Jul;140(1):232-241. doi: 10.1016/j.jaci.2016.09.035. Epub 2016 Dec 20. PubMed PMID: 28011069; PubMed Central PMCID: PMC5471135.
  • 10.Hashimoto-Tane A, Sakuma M, Ike H, Yokosuka T, Kimura Y, Ohara O, Saito T.:
    "Micro-adhesion rings surrounding TCR microclusters are essential for T cell activation."
    J Exp Med. 2016 Jul 25;213(8):1609-25. doi: 10.1084/jem.20151088. Epub 2016 Jun 27. PubMed PMID: 27354546; PubMed Central PMCID: PMC4986522.

Related Links

Lab Members

Principal investigator

Osamu Ohara
Group Director

Core members

Junshi Yazaki
Senior Scientist
Takaho Endo
Senior Scientist
Reiko Kuwahara
Technical Staff I

Contact Information

1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama City, Kanagawa,
230-0045, Japan

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