RIKEN Center for Integrative Medical Sciences Laboratory for Epigenome Inheritance
Team Leader: Azusa Inoue (Ph.D.)
Research Summary
Our laboratory is aimed to understand the inheritance mechanisms of epigenetic information across generations and to create new diagnostic and preventive strategies for disease predisposition. We study how environmental stresses in the parental generation lead to disease development in the next generation via epigenetic mechanisms in germline cells, and in particular, how these changes are transmitted to the next generation by overcoming genome-wide reprogramming that occurs immediately after fertilization. In addition, we contribute to the development of epigenetic research in Japan through the development and application of low-input epigenome analysis technologies.
Main Research Fields
- Biology
Related Research Fields
- Medicine, Dentistry & Pharmacy
- Molecular biology-related
- Embryonic medicine and pediatrics-related
- Animal life science-related
Keywords
- Epigenetics
- Nutritional metabolism
- Low-input epigenome technology
- Fetal programming
- Zygote
Selected Publications
Papers with an asterisk(*) are based on research conducted outside of RIKEN.
- 1.
Inoue A.:
"Noncanonical imprinting: Intergenerational epigenetic inheritance mediated by Polycomb complexes."
Curr Opin Genet Dev. 78:102015, 1-9 (2023) - 2.
Hayashi R, Inoue A.:
"Low-input CUT&RUN for Mouse Oocytes and Preimplantation Embryos."
Methods Mol Biol 2577:83-92 (2022) - 3.
Matoba S, Kozuka C, Miura K, Inoue K, Kumon M, Hayashi R, Ohhata T, Ogura A, Inoue A.:
"Noncanonical imprinting sustains embryonic development and restrains placental overgrowth"
Genes Dev. 36:483-494. (2022) - 4.
Mei H, Kozuka C, Hayashi R, Kumon M, Koseki H, Inoue A.:
"H2AK119ub1 guides maternal inheritance and zygotic deposition of H3K27me3 in mouse embryos."
Nat Genet. 53:539-550. (2021) - 5.
*Inoue A, Chen Z, Yin Q, Zhang Y.:
"Maternal Eed knockout causes loss of H3K27me3 imprinting and random X inactivation in the extraembryonic cells."
Genes Dev. 32, 1525-36. (2018) - 6.
*Inoue A, Jiang L, Lu F, Zhang Y.:
"Genomic imprinting of Xist by maternal H3K27me3."
Genes Dev. 31, 1927-32. (2017) - 7.
*Inoue A, Jiang L, Lu F, Suzuki T, Zhang Y.:
"Maternal H3K27me3 controls DNA methylation-independent imprinting."
Nature 547(7664), 419-24. (2017) - 8.
*Inoue A, Shen L, Matoba S, Zhang Y.:
"Haploinsufficiency, but not defective 5mC oxidation in zygotes, accounts for the developmental defects of maternal Tet3 knockouts."
Cell Reports 10(4), 763-70.(2015) - 9.
*Inoue A, Zhang Y.:
"Nucleosome assembly is required for nuclear pore complex assembly in mouse zygotes."
Nat Struct Mol Biol. 21(7), 609-16. (2014) - 10.
*Inoue A, Zhang Y.:
"Replication-dependent loss of 5-hydroxymethylcytosine in mouse preimplantation embryos"
Science 334(6053), 194. (2011)
Recent Research Results
Related Links
- Laboratory for Epigenome Inheritance (Azusa Inoue Lab)
- 2019-2023 Grant-in-Aid for Scientific Research on Innovative Areas
Program of totipotency: From decoding to designing
Lab Members
Principal investigator
- Azusa Inoue
- Team Leader
Core members
- Chisayo Kozuka
- Research Scientist
- Hailiang Mei
- Research Scientist
- Hiroto Fukushima
- JSPS PD Researcher
- Marino Maemura
- JSPS RPD Researcher
- Masahiro Matsuwaka
- Junior Research Associate
- Mami Kumon
- Technical Staff II
- Yuto Sekioka
- Student Trainee
Contact Information
1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama City, Kanagawa,
230-0045, Japan
Tel: +81-(0)45-503-7068
Email: azusa.inoue [at] riken.jp