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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1259.05c
Gene ID 01/B05
Gene synonyms/obsolete cox9
Gene product cytochrome c oxidase activity (subunit VIIA); respiratory chain complex IV (sensu Eukarya)
Entry clone Cloned
ORF length (unspliced) 239
ORF length (spliced) 177
Entry clone length 239
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1259.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTGTCGGTCCTGTAAC
Rev primer name SPCC1259.05.Rv
Rev primer SEQ AGAAAGCTGGGTATTCTGCATTTTTCTCAGCA
Amino acid length 58
Molecular weight 6.6
Isoelectric point (calc.) 9.6
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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