Gene name |
SPAC30.11 |
Gene ID |
02/C02 |
Gene synonyms/obsolete |
SPAC29E6.07 |
Gene product |
very hypothetical
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
351 |
ORF length (spliced) |
|
Entry clone length |
351 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC30.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAATACTATATTCGTGA |
Rev primer name |
SPAC30.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGCTATCTAACCAAGTAGTT |
Amino acid length |
116 |
Molecular weight |
13.8 |
Isoelectric point (calc.) |
10.4 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKGHMTQLSL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |