Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC830.09c
Gene ID 03/D06
Gene synonyms/obsolete
Gene product RNase P and RNase MRP subunit; involved in RNA processing
Entry clone Cloned
ORF length (unspliced) 420
ORF length (spliced)
Entry clone length 420
No. of intron 0
Sequence status Finished
Sequence results 333A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC830.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTTCGCTTCAAATCCCG
Rev primer name SPCC830.09.Rv
Rev primer SEQ AGAAAGCTGGGTAAACTTCAATAGGTTCATCC
Amino acid length 139
Molecular weight 15.6
Isoelectric point (calc.) 10
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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