| Gene name |
SPAC1071.08 |
| Gene ID |
03/E03 |
| Gene synonyms/obsolete |
rpp203; rpp2-3; rla6;
ysprla6 |
| Gene product |
ubiquinol cytochrome c
reductase complex subunit (cytochrome bc1 complex);
respiratory complex III |
| Entry clone |
Cloned |
| ORF length (unspliced) |
427 |
| ORF length (spliced) |
333 |
| Entry clone length |
427 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
82T:C / 313T:C |
| Comments |
Splicing pattern may
be changed by a PCR error. |
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1071.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGTATTTGGCTGCTTA |
| Rev primer name |
SPAC1071.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCGAACAAGCCGAATCCC |
| Amino acid length |
110 |
| Molecular weight |
11.1 |
| Isoelectric point (calc.) |
3.7 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
| Microscope used for
observation |
DeltaVision |