| Gene name |
SPBC83.17 |
| Gene ID |
03/H03 |
| Gene synonyms/obsolete |
|
| Gene product |
yeast mbf1 homolog;
transcription factor; multiprotein bridging factor |
| Entry clone |
Cloned |
| ORF length (unspliced) |
447 |
| ORF length (spliced) |
|
| Entry clone length |
447 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
128A:T / 129A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC83.17.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGATTGGGATACCGT |
| Rev primer name |
SPBC83.17.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTCTTTTTAGGACCGCCC |
| Amino acid length |
148 |
| Molecular weight |
15.9 |
| Isoelectric point (calc.) |
11.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSKMERALGI |
| Localization (YFP) |
nucleolus>nucleus=cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>cytosol) |
| Microscope used for
observation |
Leica |