Gene name |
SPBC19C2.14 |
Gene ID |
03/H04 |
Gene synonyms/obsolete |
smd3 |
Gene product |
probable small nuclear
ribonucleoprotein Sm D3; complexed with Cdc5p |
Entry clone |
Cloned |
ORF length (unspliced) |
447 |
ORF length (spliced) |
294 |
Entry clone length |
447 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
440G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC19C2.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTCTGTGTATTAAGTT |
Rev primer name |
SPBC19C2.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCGTCGTCCACGTGTAGGT |
Amino acid length |
97 |
Molecular weight |
11 |
Isoelectric point (calc.) |
9.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |