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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC19C2.14
Gene ID 03/H04
Gene synonyms/obsolete smd3
Gene product probable small nuclear ribonucleoprotein Sm D3; complexed with Cdc5p
Entry clone Cloned
ORF length (unspliced) 447
ORF length (spliced) 294
Entry clone length 447
No. of intron 3
Sequence status Finished
Sequence results 440G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC19C2.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTCTGTGTATTAAGTT
Rev primer name SPBC19C2.14.Rv
Rev primer SEQ AGAAAGCTGGGTATCGTCGTCCACGTGTAGGT
Amino acid length 97
Molecular weight 11
Isoelectric point (calc.) 9.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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