Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC688.15
Gene ID 05/A10
Gene synonyms/obsolete bet5; SPAC3G9.16c
Gene product May play a role in vesicular transport from endoplasmic reticulum to Golgi; Similarity Belongs to the TRAPP small subunits family; BET5 subfamily.
Entry clone Cloned
ORF length (unspliced) 515
ORF length (spliced) 468
Entry clone length 515
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC688.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTATTTATGCCTTTTA
Rev primer name SPAC688.15.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAGCAGCTTAACGTTCTC
Amino acid length 155
Molecular weight 18.3
Isoelectric point (calc.) 7.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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