Gene name |
SPBC11G11.04 |
Gene ID |
06/D05 |
Gene synonyms/obsolete |
trs20 |
Gene product |
TRAPP; Sedlin
subfamily; involved in intracellular protein transport; human
disease X-linked spondyloepiphyseal dysplasia tarda ortholog;
similar to S. cerevisiae YBR254C |
Entry clone |
Cloned |
ORF length (unspliced) |
587 |
ORF length (spliced) |
411 |
Entry clone length |
587 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
503G:A / 533T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC11G11.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACAGCATATGCAGCAAT |
Rev primer name |
SPBC11G11.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAGATACCTTCTAGCAATT |
Amino acid length |
136 |
Molecular weight |
16 |
Isoelectric point (calc.) |
7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |