| Gene name |
SPAC1B3.12c |
| Gene ID |
06/E09 |
| Gene synonyms/obsolete |
rpb10 |
| Gene product |
DNA-directed RNA
polymerase (I, II, and III subunit); involved in transcription
from Pol I promoter; involved in transcription from Pol II
promoter; involved in transcription from Pol III promoter;
DNA-directed RNA polymerase activity (function of complex);
localization DNA-directed RNA polymerase I complex;
localization DNA-directed RNA polymerase II, core complex;
abc10-beta; interacts physically with Rpb3p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
595 |
| ORF length (spliced) |
216 |
| Entry clone length |
595 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC1B3.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATCATTCCTATTCGCTG |
| Rev primer name |
SPAC1B3.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGATTCTTTTGTTTTGAC |
| Amino acid length |
71 |
| Molecular weight |
8.2 |
| Isoelectric point (calc.) |
7.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
dots at nucleolus;
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |