Gene name |
SPAC17G8.03c |
Gene ID |
06/F04 |
Gene synonyms/obsolete |
|
Gene product |
involved in
transcriptional regulation; DNA-binding protein; basal
inhibitor of class II transcription; possibly binds to TBP and
represses RNAP II during assembly of the pre-initiation
complex |
Entry clone |
Cloned |
ORF length (unspliced) |
600 |
ORF length (spliced) |
|
Entry clone length |
600 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC17G8.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGTGATCCAACAAATAA |
Rev primer name |
SPAC17G8.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCATCACCAGACGCGGAA |
Amino acid length |
199 |
Molecular weight |
22.2 |
Isoelectric point (calc.) |
5.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|