| Gene name |
SPAC2C4.13 |
| Gene ID |
06/F05 |
| Gene synonyms/obsolete |
vma16 |
| Gene product |
ATP synthase subunit;
V-type ATPase subunit; vacuolar proton pump component; V0
sector; proteolipid subunit (3rd) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
600 |
| ORF length (spliced) |
|
| Entry clone length |
600 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC2C4.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTTGTTTAGCACATC |
| Rev primer name |
SPAC2C4.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGAAAAATCACTAGCTTTT |
| Amino acid length |
199 |
| Molecular weight |
20.8 |
| Isoelectric point (calc.) |
6.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
5 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFGLIVGLLI |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|