Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC4B3.06c
Gene ID 06/G01
Gene synonyms/obsolete
Gene product NADPH-dependent FMN reductase
Entry clone Cloned
ORF length (unspliced) 603
ORF length (spliced)
Entry clone length 603
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC4B3.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACCTTGCCTGAAAAATT
Rev primer name SPCC4B3.06.Rv
Rev primer SEQ AGAAAGCTGGGTAGGGTTTCCTTTCAGGATTG
Amino acid length 200
Molecular weight 22.1
Isoelectric point (calc.) 8.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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