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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC63.12c
Gene ID 06/H06
Gene synonyms/obsolete
Gene product 20S proteasome component (beta 3)
Entry clone Cloned
ORF length (unspliced) 615
ORF length (spliced)
Entry clone length 615
No. of intron 0
Sequence status Finished
Sequence results 579A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC63.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTATTATGGAATATAA
Rev primer name SPCC63.12.Rv
Rev primer SEQ AGAAAGCTGGGTAATCTTGGCGACCCTTAACA
Amino acid length 204
Molecular weight 22.6
Isoelectric point (calc.) 4.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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