Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPACUNK4.11c
Gene ID 07/A07
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan; predicted coiled-coil region
Entry clone Cloned
ORF length (unspliced) 622
ORF length (spliced) 567
Entry clone length 622
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPACUNK4.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGTCTAAACTTCTTTC
Rev primer name SPACUNK4.11.Rv
Rev primer SEQ AGAAAGCTGGGTAGTTGCGTTTTTTCTTCTTT
Amino acid length 188
Molecular weight 21.5
Isoelectric point (calc.) 10
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 150
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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