Gene name |
SPAC19D5.05c |
Gene ID |
07/C11 |
Gene synonyms/obsolete |
|
Gene product |
ribonucleoprotein
complex; processome component; involved in rRNA processing; U3
snoRNP component; involved in rRNA processing |
Entry clone |
Cloned |
ORF length (unspliced) |
637 |
ORF length (spliced) |
552 |
Entry clone length |
637 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC19D5.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGAATTTTAAAGCACCG |
Rev primer name |
SPAC19D5.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAGAAGATCATAATCATCA |
Amino acid length |
183 |
Molecular weight |
21.7 |
Isoelectric point (calc.) |
9.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |