| Gene name |
SPBC32F12.13c |
| Gene ID |
07/D01 |
| Gene synonyms/obsolete |
SPBC19C7.01 |
| Gene product |
sequence orphan;
hypothetical protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
639 |
| ORF length (spliced) |
591 |
| Entry clone length |
639 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC32F12.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAACCAATGGAAGTTT |
| Rev primer name |
SPBC32F12.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTCTTTTTCCTTGTATCCA |
| Amino acid length |
196 |
| Molecular weight |
22.5 |
| Isoelectric point (calc.) |
10.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEKQMKKLQL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>cytosol) |
| Microscope used for
observation |
Zeiss |