Gene name |
SPAC22A12.12c |
Gene ID |
08/E12 |
Gene synonyms/obsolete |
|
Gene product |
exosome (RNase
complex); involved in rRNA processing |
Entry clone |
Cloned; Mixture |
ORF length (unspliced) |
723 |
ORF length (spliced) |
|
Entry clone length |
723 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
Mixture |
Comments |
Mixture of 2 clones,
one of which is frameshifted from somewhere. |
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC22A12.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTGAAGTTGAAGAAAA |
Rev primer name |
SPAC22A12.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGAGCTTTTTAATAAGGTCT |
Amino acid length |
240 |
Molecular weight |
26.7 |
Isoelectric point (calc.) |
7.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
no apparent
signal |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
Zeiss |