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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC530.07c
Gene ID 08/F11
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 729
ORF length (spliced)
Entry clone length 729
No. of intron 0
Sequence status Finished
Sequence results 251A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC530.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATCCTTATACAGTTGC
Rev primer name SPBC530.07.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCCTTTTCAAACGCGGTA
Amino acid length 242
Molecular weight 27.8
Isoelectric point (calc.) 5.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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