Gene name |
SPCC297.04c |
Gene ID |
09/C11 |
Gene synonyms/obsolete |
|
Gene product |
SET domain; histone
lysine methyltransferase; conserved in archaea; no apparent Sc
ortholog |
Entry clone |
Cloned# |
ORF length (unspliced) |
757 |
ORF length (spliced) |
444 |
Entry clone length |
757 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC297.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGGATTCCTGTTATTCG |
Rev primer name |
SPCC297.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAAACTAATATTTTGTAAG |
Amino acid length |
147 |
Molecular weight |
17.2 |
Isoelectric point (calc.) |
4.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |