Gene name |
SPAC19A8.10 |
Gene ID |
09/D11 |
Gene synonyms/obsolete |
|
Gene product |
zinc finger protein;
zf-C3HC4 type (RING finger); ubiquitin ligase (E3); similar to
Sp SPAC343.18 (paralog); no apparent orthologs |
Entry clone |
Cloned
(Re-cloned) |
ORF length (unspliced) |
765 |
ORF length (spliced) |
|
Entry clone length |
765 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC19A8.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAATTTAATGGAAGCAA |
Rev primer name |
SPAC19A8.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATAAAATAATTCCCACATG |
Amino acid length |
254 |
Molecular weight |
28.9 |
Isoelectric point (calc.) |
8.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nuclear dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |