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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC24B10.20
Gene ID 09/E03
Gene synonyms/obsolete
Gene product hypothetical short chain dehydrogenase
Entry clone Cloned
ORF length (unspliced) 765
ORF length (spliced)
Entry clone length 765
No. of intron 0
Sequence status Finished
Sequence results 325A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC24B10.20.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGGCTGCTGGTGGGAT
Rev primer name SPCC24B10.20.Rv
Rev primer SEQ AGAAAGCTGGGTAAAATGGAAGTTTTGTGCCA
Amino acid length 254
Molecular weight 27.7
Isoelectric point (calc.) 8.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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