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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC8E11.10
Gene ID 09/E05
Gene synonyms/obsolete sou1
Gene product sorbitol utilization protein; short chain dehydrogenase; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 768
ORF length (spliced)
Entry clone length 768
No. of intron 0
Sequence status Finished
Sequence results 595T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC8E11.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTTCTATGTTTTCTCT
Rev primer name SPAC8E11.10.Rv
Rev primer SEQ AGAAAGCTGGGTAACGAGAACAGTAACCACCG
Amino acid length 255
Molecular weight 27.4
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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