| Gene name |
SPBC336.08 |
| Gene ID |
09/F02 |
| Gene synonyms/obsolete |
|
| Gene product |
spindle pole body
protein; similar to S. cerevisiae YMR117C; coiled-coil;
centromere component (GFP) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
774 |
| ORF length (spliced) |
597 |
| Entry clone length |
774 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC336.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAGACAGTCCAATTGA |
| Rev primer name |
SPBC336.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTTTGTCCTTACTATCA |
| Amino acid length |
198 |
| Molecular weight |
23 |
| Isoelectric point (calc.) |
4.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol; SPB |
| Comments for localization |
cytoplasmic dots by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |