Gene name |
SPCC330.05c |
Gene ID |
10/A08 |
Gene synonyms/obsolete |
ura4 |
Gene product |
orotidine 5'-phosphate
decarboxylase activity; regulated by the SAPK pathway;
transcriptionally regulated by glucose |
Entry clone |
Cloned |
ORF length (unspliced) |
795 |
ORF length (spliced) |
|
Entry clone length |
795 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC330.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGCTAGAGTATTTCA |
Rev primer name |
SPCC330.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATGCTGAGAAAGTCTTTGC |
Amino acid length |
264 |
Molecular weight |
29.3 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|