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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC16A11.13
Gene ID 10/A09
Gene synonyms/obsolete luc7
Gene product U1 snRNA-associated protein; involved in mRNA splicing; predicted coiled-coil region
Entry clone Cloned
ORF length (unspliced) 795
ORF length (spliced)
Entry clone length 795
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC16A11.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCCGCTGAACAGAGGAA
Rev primer name SPCC16A11.13.Rv
Rev primer SEQ AGAAAGCTGGGTATGCAACAAAGGAAGTTGAG
Amino acid length 264
Molecular weight 30.9
Isoelectric point (calc.) 6.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss, DeltaVision

Image information
YFP 3 images) See all images

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