Gene name |
SPAC17G6.09 |
Gene ID |
10/F01 |
Gene synonyms/obsolete |
sec62 |
Gene product |
SRP receptor activity;
involved in intracellular protein transport; involved in
protein-ER targeting; involved in SRP-dependent
cotranslational membrane targeting |
Entry clone |
Cloned# |
ORF length (unspliced) |
822 |
ORF length (spliced) |
|
Entry clone length |
822 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC17G6.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTCTTCTAATGTACC |
Rev primer name |
SPAC17G6.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCAGAGACTTCTTCGACG |
Amino acid length |
273 |
Molecular weight |
31.5 |
Isoelectric point (calc.) |
10.7 |
Signal SEQ |
|
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFALVVLALVL |
Localization (YFP) |
ER |
Comments for localization |
accumulated ER by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |