Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC11C11.05
Gene ID 11/C08
Gene synonyms/obsolete
Gene product involved in cell wall biosynthesis
Entry clone Cloned
ORF length (unspliced) 855
ORF length (spliced)
Entry clone length 855
No. of intron 0
Sequence status Finished
Sequence results 478T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC11C11.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAACCACCATGTTGAT
Rev primer name SPBC11C11.05.Rv
Rev primer SEQ AGAAAGCTGGGTAGGAATATAACAACCCACGT
Amino acid length 284
Molecular weight 31.1
Isoelectric point (calc.) 5.7
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER; Golgi
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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