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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1347.09
Gene ID 11/C09
Gene synonyms/obsolete
Gene product methyltransferase; hexaprenyldihydroxybenzoate methyltransferase activity
Entry clone Cloned
ORF length (unspliced) 855
ORF length (spliced)
Entry clone length 855
No. of intron 0
Sequence status Finished
Sequence results 659T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1347.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGGATATAGTTTATGC
Rev primer name SPBC1347.09.Rv
Rev primer SEQ AGAAAGCTGGGTATGTTTTGATACCACTAATA
Amino acid length 284
Molecular weight 31.7
Isoelectric point (calc.) 6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>=cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 2 images) See all images

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