Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAP27G11.15
Gene ID 11/C11
Gene synonyms/obsolete
Gene product Slx-like structure-specific endonuclease subunit; Holliday junction resolvase; endo/exonuclease amino terminal domain; involved in DNA repair involved in meiotic recombination; involved in the processing of stalled and collapsed replication forks
Entry clone Cloned
ORF length (unspliced) 857
ORF length (spliced) 816
Entry clone length 857
No. of intron 1
Sequence status Finished
Sequence results 439G:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAP27G11.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATTTGTGCAATTTCTA
Rev primer name SPAP27G11.15.Rv
Rev primer SEQ AGAAAGCTGGGTATTTTTCTAGTTCTAGATCT
Amino acid length 271
Molecular weight 31.7
Isoelectric point (calc.) 8.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Leica

Image information
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