Gene name |
SPAC29A4.14c |
Gene ID |
11/C12 |
Gene synonyms/obsolete |
|
Gene product |
peroxin; involved in
peroxisome assembly |
Entry clone |
Cloned |
ORF length (unspliced) |
1204 |
ORF length (spliced) |
1041 |
Entry clone length |
1204 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
ORF prediction was
changed. |
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC29A4.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTAAAATCGTTTGAAAA |
Rev primer name |
SPAC29A4.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACAATCATCAAAACTGGAA |
Amino acid length |
346 |
Molecular weight |
39.4 |
Isoelectric point (calc.) |
4.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |