Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2F7.15
Gene ID 11/E11
Gene synonyms/obsolete
Gene product mitochondrial ribosomal protein small subunit
Entry clone Cloned
ORF length (unspliced) 865
ORF length (spliced) 777
Entry clone length 865
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC2F7.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTTTTAATCCCCTTTT
Rev primer name SPAC2F7.15.Rv
Rev primer SEQ AGAAAGCTGGGTATTCATCTGTAGTTGCTATC
Amino acid length 258
Molecular weight 29.8
Isoelectric point (calc.) 9.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.