Gene name |
SPAC6F12.07 |
Gene ID |
11/F06 |
Gene synonyms/obsolete |
|
Gene product |
mitochondrial outer
membrane translocase complex; involved in mitochondrial outer
membrane protein import; mitochondrial import receptor;
involved in the organization of Tom40p dimers into TOM
structures |
Entry clone |
Cloned |
ORF length (unspliced) |
867 |
ORF length (spliced) |
459 |
Entry clone length |
867 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC6F12.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGGCGATCAGTTATTAT |
Rev primer name |
SPAC6F12.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCGACGGAAGGAATGCTT |
Amino acid length |
152 |
Molecular weight |
17.5 |
Isoelectric point (calc.) |
9.7 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
24 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |