Gene name |
SPAC323.05c |
Gene ID |
11/F09 |
Gene synonyms/obsolete |
|
Gene product |
methyltransferase;
adenine-specific DNA methylase |
Entry clone |
Cloned |
ORF length (unspliced) |
867 |
ORF length (spliced) |
696 |
Entry clone length |
867 |
No. of intron |
4 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC323.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTTCGACCCCAGTTAC |
Rev primer name |
SPAC323.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCATCCCAAATTGTATTA |
Amino acid length |
231 |
Molecular weight |
25.5 |
Isoelectric point (calc.) |
4.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSTPVTSQLRL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |